Screening of protein-protein interactions between viruses and the brown planthopper, Nilaparvata lugens.

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Many viral diseases of plants are transmitted by hemipterans. These hemipteran insects are small in size (1-4 mm) but their reproductive capacity is very high. This characteristic allows insects to get tolerance for pesticides. Recently, planthoppers acquired resistance against some pesticides and virus diseases of rice plants became serious problem in Asia. To prevent virus transmission by these insects, we have to understand how viruses utilize molecules in vector insects to be transmitted by them. However, molecular interactions between insects and the transmitted viruses are not understood well. To reveal these interactions, we searched protein-protein interactions between the brown planthopper, Nilaparvata lugens, and viruses transmitted by the insect. Yeast two hybrid analyses detected many planthopper candidate proteins which may be interact in insect body.