Detection and analysis of invasive insect populations based on mtDNA sequences of stored and newly collected specimens

日本天牛的遗传变异。根据线粒体DNA序列进行了检测。利用从日本大陆、琉球群岛和台湾的75个地点获得的储存标本和新收集的样品，对两个片段进行了测序，其中一个1.2kb长的片段包含细胞色素氧化酶基因，另一个1.4kb长的片段包含rDNA。在基于序列的系统发育分析中，294个个体被分为两大类群，然后又分为7个亚类群。在A组中，密切相关的A1、A2和A3亚群主要分布在日本大陆，并大致分散在不同的地理区域，尽管A3的范围从九州扩展到琉球群岛中部。从采集于本州东部的一个个体中检测到的A4序列与中国大陆上的星星目的序列相同。在B组，B1和B2亚群分别局限于琉球群岛中部和南部，而B3在这两个地区广泛分布。这些结果表明，该甲虫的当地种群受到昆虫运动的干扰，包括由人类活动引起的昆虫运动。

Genetic variation of a Japanese longicorn beetle Anoplophora spp. was examined based on mitochondrial DNA sequences. Using stored specimens and newly collected samples obtained from 75 localities in the Japanese mainland, the Ryukyu Islands, and Taiwan, two fragments, a 1.2 kb-long fragment containing the cytochrome oxidase genes, and a 1.4 kb-long one containing rDNAs, were sequenced. In phylogenetic analyses based on the sequences, 294 individuals were divided into two major groups and then split into seven subgroups. In Group A, the closely related subgroups A1, A2, and A3 were mainly distributed on the Japanese mainland and were roughly separated among geographic areas, although the range of A3 spread from Kyushu to the Central Ryukyu Islands. The sequence of A4, detected from one individual collected in eastern Honshu, was the same as that of Anoplophora on the Chinese continent. In Group B, subgroups B1 and B2 were restricted to the Central and Southern Ryukyu Islands, respectively, while B3 was distributed widely in both regions. These results suggested that local populations of this beetle were disturved by insect movements including those caused by human activities.