Analysis of skeletal muscle fiber type transition by fatty acids using isolated fiber culture system

骨骼肌纤维根据颜色、代谢和收缩特性分为慢型和快型。在这里，我们测试了食物成分是否会影响肌纤维类型。在这里，我们成功地建立了大鼠肌纤维培养实验，其中纤维表达成熟的肌原纤维蛋白。然后，我们发现，当将富含鱼油的二十碳五烯酸（EPA）添加到纤维培养物中24小时时，PDK4和UCP3 mrna （ppar δ的已知靶基因，在慢型纤维中丰富）被上调。

Skeletal muscle fibers are classified as slow-type and fast-type based on color, metabolism and contractile properties. Here, we tested whether food component could affect muscle fiber type. Here, we succeeded in establishing a rat muscle fiber-culture assay, in which fibersexpress mature myofibrillar proteins. Then, we revealed that PDK4 and UCP3 mRNAs, known target genes of PPARδand abundant in slow-type fibers, were up-regulated when eicosapentaenoic acid (EPA), abundant in fish oil, was added to fiber cultures for 24 hours.