Development of the analytical method for estrogen-o-quinones and its application to the study on the initiation mechanism of human breast cancer

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Selective and sensitive analytical method for estrogen-o-quinones derived from catechol estrogen with enzymatic or non-enzymatic manner was developed based on the use of phenazine derivatization and LC-MS. The present method enabled to determine the catalytic activity of protein and some metal ions on the process of oxidation of catechol estrogens to the corresponding o-quinones. Furthermore, analytical method for the glutathione and L-cysteine conjugates of these o-quinones was established as one of the model for determining protein adducts of o-quinones.