Development of new sampling technique of body odors and skin emanations for diagnostic analysis

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Noninvasive sampling and sensitive analytical methods for thedetermination of the odors and emanations released from human body were developed byfiber solid-phase microextraction (SPME)/gas chromatography-mass spectrometry(GC-MS) or in-tube SPME/liquid chromatography-mass spectrometry (LC-MS). Body odorcomponents, such as volatile aldehydes and sulfur compounds, in skin emissions andsecretions were analyzed by fiber SPME/GC-MS. Using this method, we examined theeffect of smoking and the deodorant effect by interaction with 2-nonenal and antioxidantfoods. On the other hand, cortisol, testosterone and dehydroepiandrosterone in saliva wereanalyzed by automated on-line in-tube SPME/LC-MS, and used for the evaluation ofexperimental stress and fatigue. In addition, in-tube SPME/LC-MS method was used forthe analysis of 8-isoprostane and 8-OHdG as an oxidative stress biomarker in urinesamples, and applied to examine the effect of smoking.