Development of the nucleic acid aptamer for anti HIV drug

艾滋病（获得性免疫缺陷综合征）是由人类免疫缺陷病毒（HIV）引起的传染病。艾滋病（AIDS）是由免疫缺陷引起的机会性感染或恶性肿瘤的综合征。由于艾滋病病毒容易引起变异，出现耐药病毒才是严重的问题。本研究试图开发一种能与HIV蛋白酶特异性结合的核酸适体，并将其应用于抗HIV药物的研究中，构建一种野生型和两种突变型HIV蛋白酶。利用纯化的酶和SELEX方法，提出了开发与HIV蛋白酶具有亲和性的适体的可能性。

AIDS (acquired immunodeficiency syndrome) is infectious disease caused by HIV (human immunodeficiency virus). AIDS is the syndrome that developed the opportunistic infection or malignant tumor by immunodeficiency. Because HIV is easy to cause mutations, the appearance of the drug-resistant virus is the serious problem. Thus, the development of the new anti HIV drug from a different point of view is demanded.In this research, I tried to develop a nucleic acid aptamer, which specifically binds to HIV protease, and apply it to an anti HIV drug.I could construct a wild type and 2 kinds of mutant HIV proteases in this research. And the possibility of the development of aptamer, which has affinity to HIV proteases, was suggested by using purified enzymes and SELEX method.