Regulation of expression of K+ channel by degradation

点击翻译按钮获取中文摘要

We examined the regulation of degradation of strongly inwardly rectifying potassium channel (Kir2.1), using new techniques of fluorescent proteins, i.e., SNAP-tag and fluorescent timer (FT). We found the half-life of Kir2.1 changes depending on the expression level, namely current level. Patch-clamp recording showed that the cultivation under the blockade of the channel increased the whole cell conductance of Kir2.1. These results suggest the homeostatic degradation of Kir2.1 in the 293T cells, and the usefulness of fluorescence-based methods for examining the degradation of ion channels.