Biological implication of microRNAs in tumorigenesis, tumor development, and progression ? analysis with in situ hybridization using histopathological sections

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Although we examined various tumors including esophageal cancer (squamous cell carcinoma), gastric cancer (adenocarcinoma), ovarian cancer (adenocarcinoma), and hepatocellular carcinoma in this study, no apparent biological interrelationship between microRNAs and their targeted specific proteins was revealed except esophageal cancer. We supposed that the central reason for the failure to detect the interrelationship was deterioration of ISH probes and unexpected change of company that supplied them. On the contrary, we found that microRNA 199a, Brm, and Egr1 constructed double-negative feed in esophageal cancer, suggesting that they were mutually related in the process of esophageal carcinogenesis, cancer development, and its progression. But no special relationship was found between them and tumor stage. We are continuously studying about other tumors both epithelial and nonepithelial and various precancerous lesions because this research method is very unique.