Functional analysis of motility-related genes of Trypanosoma brucei: relationship with cellular morphology and apoptosis

布氏锥虫运动相关基因的功能分析：与细胞形态和细胞凋亡的关系

• 批准号: 22590380
• 负责人: TAKASHI Suzuki
• 金额: $ 2.33万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22590380/
• 关键词: アポトシス; 鞭毛; アフリカトリパノソーマ原虫; トリパノソーマ; 動き; 細胞形態変化

Double knock-down analysis of TbUNC119 and its binding molecule showed cellular apoptosis, morphological change (extended cell shape), and reduced motility in insect-form trypanosome cells. Among those phenotypes, extended cell shape was firstly observed. This was revealed to be at least in part by up-regulated endocytosis. In bloodstream-form trypanosome cells, TbUNC119 was expressed in flagellum and an area around flagella pocket, only in which endocytosis occurred. Functions of TbUNC119 and its binding protein were thus considered to include regulation of cellular endocytosis.

TbUNC119及其结合分子的双敲低分析显示昆虫型锥虫细胞中的细胞凋亡、形态学变化（延长的细胞形状）和运动性降低。在这些表型中，首先观察到延伸的细胞形状。这被揭示为至少部分通过上调的内吞作用。在血流形式的锥虫细胞中，TbUNC119在鞭毛和鞭毛袋周围的区域中表达，仅在其中发生内吞作用。因此，认为TbUNC 119及其结合蛋白的功能包括调节细胞内吞作用。

项目成果

Trypanosome altemative oxidase, a potential therapeutic targct for sleeping sickness, is conserved among Trypanosoma brucei subspecies.

锥虫替代氧化酶是昏睡病的潜在治疗靶标，在布氏锥虫亚种中是保守的。

• 发表时间: 2010
• 期刊: Parasitology International
• 影响因子: 1.9
• 作者: Kwansa-Bentum B;Ayi I;Suzuki T,Otchere J;Kumagai T;Anyan WK;Asahi H;Akao N;Wilson MD;Boakye DA;Ohta N;鈴木高史(中村康介)
• 通讯作者: 鈴木高史(中村康介)

Molecular cloning and functional analysis of Trypanosoma brucei motility-related genes.

布氏锥虫运动相关基因的分子克隆及功能分析。

• 发表时间: 2010
• 作者: Kwansa-Bentum B;Ayi I;Suzuki T,Otchere J;Kumagai T;Anyan WK;Asahi H;Akao N;Wilson MD;Boakye DA;Ohta N;鈴木高史(中村康介);鈴木高史(Kwansa-Bentum Bethel);大橋光子(鈴木高史)
• 通讯作者: 大橋光子(鈴木高史)

Differential KineticActivities of Glycerol Kinase among African Trypanosome Species: Phylogenetc and Therapeutic Implications

非洲锥虫物种中甘油激酶的差异动力学活性：系统发育等和治疗意义

• 发表时间: 2011
• 期刊: J Vet Med Sci
• 影响因子: 1.2
• 作者: Ohashi-Suzuki M;Yabu Y;Ohshima S;Nakamura K;Kido Y;Sakamoto K;Kita K;Ohta N;Suzuki T
• 通讯作者: Suzuki T

Administrative practices of health professionals and use of artesunate-amodiaquine by community members for treating uncomplicated malaria in southern Ghana: implications for artemisinin-based combination therapy deployment

• DOI: 10.1111/j.1365-3156.2011.02833.x
• 发表时间: 2011-10-01
• 期刊: TROPICAL MEDICINE & INTERNATIONAL HEALTH
• 影响因子: 3.3
• 作者: Kwansa-Bentum, Bethel;Ayi, Irene;Ohta, Nobuo
• 通讯作者: Ohta, Nobuo

Plasmodium falciparum isolates from southern Ghana exhibit polymorphisms in the SERCA-type PfATPase6 though sensitive to artesunate in vitro.

• DOI: 10.1186/1475-2875-10-187
• 发表时间: 2011-07-11
• 期刊: Malaria journal
• 影响因子: 3
• 作者: Kwansa-Bentum B;Ayi I;Suzuki T;Otchere J;Kumagai T;Anyan WK;Osei JH;Asahi H;Ofori MF;Akao N;Wilson MD;Boakye DA;Ohta N
• 通讯作者: Ohta N