The clarification of mechanisms for cytotoxic effect of Legionella hyper-toxic mutants which are intracellular growth-deficient (Toxh mutants) on host cells.

我们的特点是嗜肺军团菌高毒性突变体的细胞内生长缺陷（Toxh突变体）的感染性和细胞毒性的宿主细胞。并在Toxh突变体中发现了mini-Tn 10：：kan插入的染色体位点和一个与突变体表型相关的基因。此外，使用对复制型L.嗜肺菌感染。

We have characterized of Legionella pneumophila hyper-toxic mutants which are intracellular growth-deficient (Toxh mutants) for infectivity and cytotoxicity to host cells. And the chromosomal loci of mini-Tn10::kan-insertion in Toxh mutants and a gene responsible for a phenotype of one of their mutants have identified. Furthermore, pathogenisity of Toxh mutants were evaluated using A/J mice, which are susceptible to replicative L. pneumophila infections.