Hyperdry human amniotic membrane is useful material for tissue engineering.: Physical, morphological properties, and safety as the new biological material.

人羊膜（AM）作为一种生物移植材料在临床上有着广泛的应用。但是，在制备、储存和灭菌方面存在一些长期存在的问题。为了解决这些问题，我们开发了超干AM (HD-AM)，使用远红外射线，空气抑制和微波，然后用g射线照射灭菌。为阐明HD-AM作为生物材料的益处，以冷冻干燥AM （FD-AM）为典型冻干方法，比较HD-AM的组织学和物理特性，评价HD-AM在裸鼠体内实验的安全性，论证HD-AM移植翼状胬肉的可行性。HD-AM保留了上皮和结缔组织的形态结构。HD-AM的透水性和筛分系数显著低于FD-AM。移植后18个月，腹腔内单层和多层HD-AM降解，未见细胞浸润。临床治疗方面，术后6个月随访未见翼状胬肉复发及结膜下纤维化再生。认为HD-AM是一种安全有效的新型生物材料，可用于临床治疗复发性翼状胬肉。

Human amniotic membrane (AM) has been used widely as graft biomaterial for a variety of clinical applications. But, there are some persistent problems related to the preparation, storage, and sterilization. To resolve these problems, we developed hyperdry AM (HD-AM) using far-infrared rays, depression of air, and microwaves and then sterilized by g-ray irradiation. To elucidate the benefit of HD-AM as biological materials, compare with the histological and physical properties of HD-AM with a freeze-dried AM (FD-AM) as typical freeze-dry methods, evaluate the safety of HD-AM in vivo experiment used nude mice, and demonstrate the feasibility of HD-AM transplant in pterygium. HD-AM has kept the morphological structure of epithelium and connective tissues. The water permeability and the sieving coefficient of HD-AM were significantly lower than that of FD-AM. At 18 months after transplanted, single and multilayers of HD-AM in the intraperitoneal cavity was degraded without any infiltrated cells. For clinical treatment, recurrence of pterygium and regrowth of the subconjunctival fibrosis were not observed during the 6-month follow-up periods after the surgery. It was proposed that HD-AM was a safe and effective new biological material for clinical use including treatment for recurrent pterygium.