Are platelet-derived / endothelial cell-derived microparticles risks for cardiovascular events in general population?

我们试图在Tanushimaru研究中登记的健康普通受试者中调查血小板源性MP （PDMP）和内皮源性MP （EMP）的临床意义。在2100名受试者中获得富含PDMP / emp的组分并冷冻保存。我们采用高灵敏度、高特异性的新型流式细胞仪进行PDMP/EMP取样和流式细胞术，以替代传统流式细胞仪的检测方法。然而，PDMP和EMP的测量结果缺乏可重复性。因此，我们尝试优化流式细胞术的程序。优化后，PDMP定量测量的重现性可接受，但EMP定量测量的程序尚未优化。

We sought to investigate the clinical significance of platelet-derived MP (PDMP) and endothelium-derived MP (EMP) in healthy general subjects who were registered in the Tanushimaru study. PDMP-/EMP-rich fraction was obtained and kept freezed in 2,100 subjects. We performed PDMP/EMP sampling and flow-cytometry using new flow cytometric machine with high sensitivity and specificity, according to the widely used method which was done using old-type of flow cytometric machine. However, the PDMP and EMP measurements were lack in the reproducibility. Thus, we tried to optimized the flow cytometric procedure. After optimization, acceptable reproducibility of quantitative PDMP measurement was obtained, but we have not yet optimized the procedure of quantitative EMP measurement.