Are platelet-derived / endothelial cell-derived microparticles risks for cardiovascular events in general population?

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We sought to investigate the clinical significance of platelet-derived MP (PDMP) and endothelium-derived MP (EMP) in healthy general subjects who were registered in the Tanushimaru study. PDMP-/EMP-rich fraction was obtained and kept freezed in 2,100 subjects. We performed PDMP/EMP sampling and flow-cytometry using new flow cytometric machine with high sensitivity and specificity, according to the widely used method which was done using old-type of flow cytometric machine. However, the PDMP and EMP measurements were lack in the reproducibility. Thus, we tried to optimized the flow cytometric procedure. After optimization, acceptable reproducibility of quantitative PDMP measurement was obtained, but we have not yet optimized the procedure of quantitative EMP measurement.