Functional expression of HLA class Ib molecules in bone marrow-derived mesenchymal stromal cells and its implication for cellular immunotherapy

我们从基因和蛋白水平研究了人骨髓间充质干细胞(BM-MSCs)中HLAIb类分子的表达。在静止期的BM-MSCs中可检测到人类白细胞抗原E和人类白细胞抗原F的转录产物，但几乎检测不到人类白细胞抗原G及其选择性剪接体。IL-10处理BM-MSCs不能诱导HLAG基因的表达。在蛋白质水平上，虽然有报道BM-MSCs在其细胞表面表达经典的HLAIa类分子，但我们没有检测到其表面表达HLAF和HLAG。未来的研究有必要评估HLAIb类分子在MSCs或来源于非骨髓组织的MSC样细胞中的表达。

We have investigated the expression of HLA class Ib molecules in human bone marrow-derived mesenchymal stromal/stem cells (BM-MSCs) at mRNA and protein levels. HLA-E and HLA-F mRNA transcripts were detected in quiescent BM-MSCs but HLA-G mRNA and its alternatively spliced forms were hardly detectable. Treatment of BM-MSCs by IL-10 did not induce mRNA expression of HLA-G. At the protein level, although BM-MSCs were reported to express classical HLA class Ia molecules on their cell surface, we could not detect surface expression of HLA-F and HLA-G. Future research is warranted to evaluate the expression of HLA class Ib molecules in MSCs or MSC-like cells derived from tissues other than bone marrow.