Identification and functional analysis of esophageal cancer-related genes by pull-down method using biotin-labeled miRNA

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In order to identify possible predictive markers, We performed the identification of miRNA related to the degree of differentiation-grade and malignancy by the profiling of esophageal cancer cell lines and esophageal cancer clinical specimens. In poorly differentiated cells, miR-29a and let-7b were overexpressed. We identified target genes of these miRNAs using in vitro pull-down method, and found some candidates of target genes related to tumor suppressor and apoptosis. Our results suggest that these miRNAs are involved in esophageal cancer progression.