Identification of the marker for cholangiocarcinoma based on the data by proteomics and tissue microarray

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We analyzed resected specimens by the proteomics approach anddetected 4 unique proteins (actinin-1,actinin-4,DJ-1,cathepsin B) out of 38 higher gene expression products compared with normal bile duct tissue. Next,we evaluated theexpression level of these 4 proteins by Western blotting method among 19 bile ductcarcinoma cell lines and characterized them into low expression cell lines and highexpression cell lines. We Finally, we performed immunohistochemical analysisutilizing tissue micro array of the consecutive 96 resected specimens of bile ductcarcinoma Among 4 proteins, high expression of DJ-1 could be a marker to suggestgood prognosis (p=0604)