Identification of the marker for cholangiocarcinoma based on the data by proteomics and tissue microarray

基于蛋白质组学和组织微阵列数据鉴定胆管癌标志物

• 批准号: 22591511
• 负责人: HIRANO Satoshi
• 金额: $ 2.91万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22591511/
• 关键词: 胆道癌; tissue micro array; プロテオミクス; tisssue micro array; 腫瘍マーカー; 生存率; TMA解析; マーカー

We analyzed resected specimens by the proteomics approach anddetected 4 unique proteins (actinin-1,actinin-4,DJ-1,cathepsin B) out of 38 higher gene expression products compared with normal bile duct tissue. Next,we evaluated theexpression level of these 4 proteins by Western blotting method among 19 bile ductcarcinoma cell lines and characterized them into low expression cell lines and highexpression cell lines. We Finally, we performed immunohistochemical analysisutilizing tissue micro array of the consecutive 96 resected specimens of bile ductcarcinoma Among 4 proteins, high expression of DJ-1 could be a marker to suggestgood prognosis (p=0604)

我们采用蛋白质组学的方法对切除的胆管组织进行分析，在38个高表达的基因产物中检测到4个独特的蛋白质（actinin-1，actinin-4，DJ-1，cathepsin B）。然后，我们用Western blotting方法检测了这4种蛋白在19个胆管癌细胞系中的表达水平，并将其分为低表达细胞系和高表达细胞系。最后，我们利用组织微阵列对连续96例胆管癌切除标本进行了免疫组化分析。在4种蛋白质中，DJ-1的高表达可能是预后良好的标志物（p=0604）

项目成果

Outcome of surgical treatment of hilar cholangiocarcinoma: a special reference to postoperative morbidity and mortality

• DOI: 10.1007/s00534-009-0208-1
• 发表时间: 2010-07-01
• 期刊: JOURNAL OF HEPATO-BILIARY-PANCREATIC SCIENCES
• 影响因子: 3
• 作者: Hirano, Satoshi;Kondo, Satoshi;Kawasaki, Ryosuke
• 通讯作者: Kawasaki, Ryosuke