Antitumor effect of Chelated Endostatin

螯合内皮抑素的抗肿瘤作用

• 批准号: 22591572
• 负责人: YANO Motoki
• 金额: $ 2.91万
• 依托单位: Nagoya City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22591572/
• 关键词: エンドスタチン; 呼吸器外科学

We have assessed antitumor effect of chelated endostatin generating vector which secret chelated endostatin. We injected Lewis lung cancer cells (1×10^6) at the lateral abdominal wall of the BALBC mice. When the produced cancer nodule became 100mm^3in a volume, we started chemotherapy using endostatin transfection and/or anticancer agents (DTX, 5-FU). Then antitumor effect was assessed. Endostatin tranfection was performed by direct injection with plasmids surrounding the cancer nodule. Unfortunately we could not find any advantages of endostatin transfection accumulating anticancer agents.

我们评价了分泌螯合内皮抑素的螯合内皮抑素生成载体的抗肿瘤作用。将刘易斯肺癌细胞（1×10^6）注射于BALB/c小鼠的侧腹壁。当产生的癌结节体积达到100 mm^3时，我们开始使用内皮抑制素转染和/或抗癌剂（DTX，5-FU）进行化疗。评价抗肿瘤作用。通过直接注射癌结节周围的质粒进行内皮抑素转染。不幸的是，我们没有发现内皮抑素转染积累抗癌药物的任何优势。