Protective effects of regulatory T cells on ischemic brain damage

调节性T细胞对缺血性脑损伤的保护作用

• 批准号: 22591590
• 负责人: SAKANAKA Masahiro
• 金额: $ 3万
• 依托单位: Ehime University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22591590/
• 关键词: 脳虚血; 制御性T細胞; FoxP3; ヒスタミン; マイクログリア; 炎症

The organic cation transporters 3 (= OCT3) was identified as an uptake-2 transporter, responsible for clearance of histamine. Because increasing evidence suggests the involvement of histamine in cerebral ischemia, we investigated the effects of targeted disruption of OCT3 on the severity of ischemic brain damage. Transient focal ischemia for 1 hour was induced by occlusion of the middle cerebral artery (MCA) of homozygous Oct3-deficient mice and their wild type littermates. Consequently, targeted disruption of OCT3 significantly increased histamine content in the ischemic cortex and significantly reduced the infarct volume after cerebral ischemia. Furthermore, this disruption prevented the reduction of regulatory T cell proportion after cerebral ischemia. Since repeated administration of L-histidine (a precursor of histamine) to wild-type mice also showed the same effects, our observations suggested that OCT3 is the molecule responsible for clearance of ischemia-induced histamine in the brain and targeted disruption of OCT3 ameliorated ischemic brain damage through an increase in regulatory T cells.

有机阳离子转运蛋白3（= OCT 3）被鉴定为摄取-2转运蛋白，负责组胺的清除。由于越来越多的证据表明组胺参与脑缺血，我们研究了OCT 3靶向破坏对缺血性脑损伤严重程度的影响。通过闭塞纯合Oct 3缺陷小鼠及其野生型同窝小鼠的大脑中动脉（MCA）诱导短暂局灶性缺血1小时。因此，OCT 3的靶向破坏显著增加了缺血皮质中的组胺含量，并显著减少了脑缺血后的梗死体积。此外，这种破坏防止了脑缺血后调节性T细胞比例的减少。由于对野生型小鼠重复给予L-组氨酸（组胺的前体）也显示出相同的效果，我们的观察结果表明OCT 3是负责清除脑中缺血诱导的组胺的分子，并且OCT 3的靶向破坏通过调节性T细胞的增加改善了缺血性脑损伤。

项目成果

Iba1+/NG2+ macrophage-like cells expressing a variety of neuroprotective factors ameliorate ischemic damage of the brain

• DOI: 10.1038/jcbfm.2009.233
• 发表时间: 2010-03-01
• 期刊: JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
• 影响因子: 6.3
• 作者: Smirkin, Anna;Matsumoto, Hiroaki;Tanaka, Junya
• 通讯作者: Tanaka, Junya

Effects of ginsenoside Rb_1 on skin changes

人参皂苷Rb_1对皮肤变化的影响

• 发表时间: 2012
• 期刊: J Biomed Biotechnol
• 作者: Kimura Y;Sumiyoshi M;Sakanaka M
• 通讯作者: Sakanaka M

Accuracy of Diffusion Tensor Magnetic Resonance Imaging-Based Tractography for Surgery of Gliomas Near the Pyramidal Tract: A Significant Correlation Between Subcortical Electrical Stimulation and Postoperative Tractography

• DOI: 10.1227/neu.0b013e31823020e6
• 发表时间: 2012-02-01
• 期刊: NEUROSURGERY
• 影响因子: 4.8
• 作者: Ohue, Shiro;Kohno, Shohei;Ohnishi, Takanori
• 通讯作者: Ohnishi, Takanori

Delayed neuronal cell death in brainstem after transient brainstem ischemia in gerbils

• DOI: 10.1186/1471-2202-11-115
• 发表时间: 2010-09-14
• 期刊: BMC NEUROSCIENCE
• 影响因子: 2.4
• 作者: Cao, Fang;Hata, Ryuji;Gyo, Kiyofumi
• 通讯作者: Gyo, Kiyofumi

Cancer stem-like cells of glioblastoma characteristically express MMP-13 and display highly invasive activity

• DOI: 10.3892/ijo_00000764
• 发表时间: 2010-11-01
• 期刊: INTERNATIONAL JOURNAL OF ONCOLOGY
• 影响因子: 5.2
• 作者: Inoue, Akihiro;Takahashi, Hisaaki;Ohnishi, Takanori
• 通讯作者: Ohnishi, Takanori