Immunotherapy with fusions of dendritic and glioma stem cells against malignant glioma

从恶性胶质瘤患者手术标本中建立的CD133+胶质瘤干细胞（GSCs）与自体树突状细胞（DCs）融合。用多核糖素多核糖素酸（dsRNA）和IL-10的siRNA （IL-10-siRNA）刺激融合细胞（fc）。用自体FCs刺激CD4+ T细胞进行ELISPOT检测，结果显示FCs可引起有效的Th1应答。本研究结果提示DCs与GSCs融合的免疫治疗可能是一种很有前途的靶向GSCs的策略。

CD133+ glioma stem cells (GSCs) established from surgical specimens of patients with malignant glioma were fused with autologous dendritic cells (DCs). Fusion cells (FCs) were stimulated with polyriboinosinic polyribocytidylic acid (dsRNA) and siRNA of IL-10 (IL-10-siRNA). CD4+ T cells stimulated with autologous FCs were subjected to the ELISPOT assay, which revealed that the FCs elicit an efficient Th1 response. The results of this study are suggesting that the immunotherapy with fusions of DCs and GSCs may be a promising strategy for targeting GSCs.