Therapeutic effects of transplanted peripheral blood mononuclear cells which mobilized by G-CSF on spinal cord injury in mice

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We analyzed that intraspinal transplantation of CD34+ peripheral blood cells mobilized by granulocyte colony-stimulating factor (G-CSF) facilitates angiogenesis in injured spinal cord and promotes functional recovery after spinal cord injury (SCI). NOD-Scid mice were subjected to contusion-induced SCI. Human CD34+ peripheral blood stem cells (1.0x10^5) mobilized by G-CSF were injected into the injured spinal cords (CD34 group) one week after SCI. Human mononuclear cell fraction (1.0x10^5) mobilized by G-CSF (M group) or saline (Control group) were also administrated. Mice in CD34 and M groups showed significant locomotor recovery of hind limb motor function fromsix to eight weeks after the transplantation (p<0.01). A certain amount of transplanted cells were detected until four weeks, and they were differentiated into vascular endothelial cells. Those results indicate that intraspinal transplantation of human peripheral blood stem cells mobilized by G-CSF facilitates the angiogenesis in injured spinal cord and promotes functional recovery in mice.