anti-VEGF neutralizing antibody inhibits the progress of ossification of the ligament in the ossification of the spinal ligament

抗VEGF中和抗体在脊柱韧带骨化中抑制韧带骨化的进展

• 批准号: 22591631
• 负责人: ASANUMA Yumiko
• 金额: $ 2.75万
• 依托单位: Mie University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22591631/
• 关键词: 脊椎脊髄病学; 脊椎靱帯骨化症; VEGF中和抗体; twy/twy マウス; 脊椎靭帯骨化症; twy/twyマウス

Tiptoe Walking (ttw) mouse is a model for ectopic ossification of various soft tissues in the extremities and spine. Ectopic ossification of spinal ligaments results in ossification of the posterior longitudinal ligament (OPLL) and of the yellow ligament (OYL) of the spine. In this study, anti-VEGF neutralizing antibody was administered intraperitoneally in ttw mouse and the ossified area of the spinal ligament was measured. The increase of the ossification of the spinal ligament was inhibited in the ttw mouse, which anti-VEGF neutralizing antibody was administered (group V), compared with the control ttw mouse, which PBS was administerd (group P). Osteocalcin immunopositive cells and expression of type X collagen were observed in the ossification front of group P, whereas safranine-O staining cells was seen and expression of type X collagen were little in that of group V, which indicates that hypertrophy of the chondrocytes and/or induction of the osteoblasts are inhibited and chondrocytes are persisted. It suggests that suppression of VEGF may inhibit the course of endochondral ossification in the spinal ligament. Vascular invasion was suppressed in the ossified area of the spinal ligament in group V compared with that in group P, which suggested that inhibition of vascular invasion by administration of anti-VEGF neutralizing antibody may also suppress the endochondral ossification in the spinal ligament.

脚尖行走(TTW)小鼠是四肢和脊柱各种软组织异位骨化的模型。脊柱韧带的异位骨化导致脊柱的后纵韧带(OPLL)和黄色韧带(OYL)的骨化。本研究用抗血管内皮生长因子中和抗体对TTW小鼠进行了体内注射，测量了小鼠脊柱韧带骨化面积。注射抗血管内皮细胞生长因子中和抗体的TTW小鼠(V组)与注射PBS的TTW小鼠(P组)相比，脊柱韧带骨化的增加受到抑制。P组在骨化前沿可见骨钙素免疫阳性细胞和X型胶原表达，而V组仅见藏红O染色细胞，X型胶原表达较少，表明软骨细胞肥大和/或成骨细胞的诱导受到抑制，软骨细胞持续存在。提示抑制血管内皮生长因子可能抑制了脊柱韧带软骨内骨化的进程。与P组相比，V组脊髓韧带骨化区的血管侵袭受到抑制，提示应用抗血管内皮生长因子中和抗体抑制血管侵袭也可能抑制脊髓韧带的软骨内骨化。

项目成果

化膿性脊椎炎において、長期間、保存療法を要した症例について

需要长期保守治疗的化脓性脊柱炎病例

• 发表时间: 2011
• 作者: 三浦良浩;淺沼由美子;田中雅;長倉剛;小川邦和
• 通讯作者: 小川邦和

硬膜外急性リンパ節白血病の1例

硬膜外急性淋巴结白血病1例

• 发表时间: 2011
• 作者: 今西隆夫;明田浩司;内藤陽平;榊原紀彦;笠井裕一;須藤啓広
• 通讯作者: 須藤啓広

Erb点刺激による上肢筋のCMAPが責任病巣の把握と予後予測に有用であった一例

使用 Erb 点刺激对上肢肌肉进行 CMAP 如何有助于识别相关病变和预测预后的示例。

• 发表时间: 2011
• 作者: Nagamoto Y;Iwasaki M;Fujimori T;淺沼由美子
• 通讯作者: 淺沼由美子

The Intradiscal Injection of Autologous Platelet-rich Plasma-serum induced the Restoration of Disk Hight in the Rabbit Anular Needle Puncture Model

椎间盘内注射自体富血小板血浆诱导兔环针穿刺模型椎间盘高度恢复

• 发表时间: 2011
• 作者: Koji Akeda;Shuji Obata;Ryo Morimoto;Yumiko Asanuma;Yuichi Kasai;Atsumasa Uchida;Akihiro Sudo
• 通讯作者: Akihiro Sudo

腰椎脊柱管外病変を示唆する臨床症状と画像所見の検討

临床症状和影像学检查提示腰椎椎管外病变

• 发表时间: 2011
• 作者: 淺沼由美子;田中雅;長倉剛;三浦良浩;小川邦和
• 通讯作者: 小川邦和