Molecular mechanisms of volatile anesthetics toxicity on the immature mamarian brain cells: Analysis by single fluorescent molecule imaging.

挥发性麻醉剂对未成熟哺乳动物脑细胞毒性的分子机制：单荧光分子成像分析。

• 批准号: 22591707
• 负责人: MIYAMOTO Yoshikazu
• 金额: $ 3万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22591707/
• 关键词: 揮発性麻酔薬; 幼若脳神経細胞障害; 分子機構; 1分子イメージング; １分子イメージング; 幼若神経細胞障害

In this study, we tried to find out the whole mechanism of the toxicity of volatile anesthetics to fetal rat brain. We speculated that volatile anesthetics might cause injury through inhibition of neurotrophins such as NGF or BDNF to bind to each receptor on the surface of the neuronal cells, or they might inhibit dimerization of the NGF/BDNF-receptor complexes.We established the single Qdot-NGF/BDNF molecule live cell imaging system, and now analyzing if volatile anesthetics affect the binding of NGF or BDNF to each receptor or dimerization of the NGF/BDNF-receptor complexes.

本研究试图从整体上探讨挥发性麻醉药对胎鼠脑毒性的机制。我们推测挥发性麻醉药可能通过抑制神经营养因子如NGF或BDNF与神经细胞表面各受体的结合或抑制NGF/BDNF-受体复合物的二聚化而引起损伤。现在分析挥发性麻醉剂是否影响NGF或BDNF与每个受体的结合或NGF/BDNF-受体复合物的二聚化。

项目成果

Stickler症候群患児の麻酔経験

Stickler综合征患儿的麻醉体会

• 发表时间: 2012
• 作者: Fukuda M;Yamamoto T;Nishimura S;Kato T;Murakami W;Hino A;Ono M;Tateishi H;Oda T;Okuda S;Kobayashi S;Koseki N;Kyushiki H;Yano M;加藤佳子，宮本善一，澁田達史，山本俊介，磐井多美子，内田整．
• 通讯作者: 加藤佳子，宮本善一，澁田達史，山本俊介，磐井多美子，内田整．

Influences of general anesthetics on the developing mammalian brain

全身麻醉对哺乳动物大脑发育的影响

• 发表时间: 2011
• 期刊: Masui
• 作者: Kimura-Kuroiwa K;Adachi YU;Mimuro S;Kawamata M;Sato S;Matsuda N.;Miyamoto Y
• 通讯作者: Miyamoto Y