The mechanism of neuronal death regulated by the mitochondria and the development of brain protection

细胞凋亡与神经元损伤密切相关。凋亡相关蛋白的切割是递增的，抓住凋亡级联反应的这一起始步骤将是研究神经元损伤的良好工具。我们将共振能量转移荧光（FRET）技术应用于原代培养的神经元死亡模型中，尝试对神经元死亡相关蛋白的裂解进行清晰成像。我们已经开发了定量分析荧光共振能量转移变化的方案，诱导的神经元培养。

Apoptosis is deeply related to neuronal injury. Apoptosis-related proteins are incrementally cleaved, and it would be a good tool for the research of neuronal injury to catch this initial step of the apoptotic cascades. We applied the technique of FRET(Fluorescence of resonance energy transfer) to the neuronal death model of primary neuronal culture, and tried to make a resolutional image of cleavage of apoptosis-related protein. We have developed the protocol of quantitative analysis of FRET change of apoptosis-induced neuronal culture.