The mechanism of neuronal death regulated by the mitochondria and the development of brain protection

细胞凋亡与神经元损伤密切相关。细胞凋亡相关蛋白是逐渐裂解的，捕捉细胞凋亡级联反应的初始阶段将是研究神经元损伤的一个很好的工具。我们将FRET（荧光共振能量转移）技术应用于原代神经元培养的神经元死亡模型，并试图获得细胞凋亡相关蛋白切割的分辨率图像。我们制定了细胞凋亡诱导的神经元培养中FRET变化的定量分析方案。

Apoptosis is deeply related to neuronal injury. Apoptosis-related proteins are incrementally cleaved, and it would be a good tool for the research of neuronal injury to catch this initial step of the apoptotic cascades. We applied the technique of FRET(Fluorescence of resonance energy transfer) to the neuronal death model of primary neuronal culture, and tried to make a resolutional image of cleavage of apoptosis-related protein. We have developed the protocol of quantitative analysis of FRET change of apoptosis-induced neuronal culture.