The mechanism of cancer cell invasion identified by micro -RNA expression at the invasive front of prostate cancer

前列腺癌侵袭前沿微小RNA表达鉴定癌细胞侵袭机制

• 批准号: 22591772
• 负责人: SATO Fuminori
• 金额: $ 2.83万
• 依托单位: Oita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22591772/
• 关键词: 前立腺癌; 浸潤; micro-RNA; invasive front; microRNA; 発現解析; マイクロRNA; 前立腺組織; microRMA

The aim of this study is to elucidate the molecular mechanism of prostate cancer cell invasion using micro-RNA (miRNA) expression analysis. Paraffin-embedded human prostate cancer tissues were used for this study. The miRNA was extracted from the invasive front and noninvasive part of cancer lesion. Individual prostate cancer expressed various miRNAs. Unexpectedly, the miRNA expression pattern at the invasive front was not significantly different from that of non-invasive part of cancer.The major limitation of this study is small sample size (n=32), and the quality of miRNA extracted from paraffin-embedded tissues. From this study, miRNA may not influence or play an important role for the prostate cancer invasion, but we cannot reach the conclusion. Further investigation should be done to answer this principal question.

本研究的目的是利用micro-RNA（miRNA）表达分析来阐明前列腺癌细胞侵袭的分子机制。石蜡包埋的人前列腺癌组织用于本研究。从癌灶的侵袭性前部和非侵袭性部分提取miRNA。个体前列腺癌表达各种miRNA。本研究的主要局限性在于样本量小（n=32），以及从石蜡包埋组织中提取的miRNA的质量。从本研究来看，miRNA可能对前列腺癌的侵袭没有影响或起重要作用，但我们还不能得出结论。要回答这个主要问题，还需要作进一步的研究。