The analysis of the hypoxia-induced regulation mechanism in erythropetin gene expression and erythrocytosis

缺氧诱导促红细胞生成素基因表达及红细胞增多的调控机制分析

• 批准号: 22790895
• 负责人: OBARA Naoshi
• 金额: $ 2.58万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22790895/
• 关键词: Notchシグナル; Nestin; エリスロポエチン; 造血不全; ストローマ細胞; 赤血球造血

Recently, the possibility has been suggested that there are bone marrow stromal cells expressing Nestin genes which are neural stem cell markers and that those cells play an important role in hematopoiesis. Therefore, we crossbred RBP-J conditional-knockout mice. RBP-J are downstream genes of Notch signaling. and NestinCre mice and analyzed the hematopoiesis of those mice with which Nestin-expressing-cell-specific RBP-J signaling was knocked out. The fetuses of the knockout mice suffered from severe anemia during embryonic stage, the differentiation of their erythroblasts was impaired and the onset of erythropoietin was significantly lowered. The erythropoietin productive cells of fetal livers developed Nestin and it was suggested that stromal cells of fetal livers may be important to hematopoietic differentiation.

最近，已经提出，有可能存在表达神经干细胞标志物Nestin基因的骨髓基质细胞，并且这些细胞在造血中发挥重要作用。因此，我们杂交RBP-J条件敲除小鼠。RBP-J是Notch信号传导的下游基因。和NestinCre小鼠，并分析Nestin表达细胞特异性RBP-J信号转导被敲除的那些小鼠的造血。基因敲除小鼠的胎儿在胚胎期患有严重贫血，其成红细胞分化受损，促红细胞生成素的产生显著降低。胎肝中的促红细胞生成素分泌细胞发育Nestin，提示胎肝基质细胞可能在造血分化中起重要作用。

项目成果

Detection of CD55-and CD59-negative immature reticulocytes may improves sensitivity/specificity to identify aminor population of PNH-type cells in patients with myelodysplastic syndrome/aplastic anemia

检测 CD55 和 CD59 阴性未成熟网织红细胞可提高识别骨髓增生异常综合征/再生障碍性贫血患者中 PNH 型细胞少数群体的敏感性/特异性

• 发表时间: 2011
• 作者: 小原直;佐藤晶子;千葉滋;二宮治彦
• 通讯作者: 二宮治彦

Outcomes of splenectomy for adult patients with ITP-a single center retrospective analysis

成人ITP患者脾切除术后的单中心回顾性分析

• 发表时间: 2011
• 作者: Yasushi Okoshi;Naoki Kurita;Hidekazu Nishikii;Yasuhisa Yokoyama;Mamiko Sakata-Yanagimoto;Naoshi Obara;Kazumi Suzukawa;Yuichi Hasegawa;Shigeru Chiba
• 通讯作者: Shigeru Chiba

発症から8年経過し再発後の治療が奏功しているT細胞性中枢神経原発

发病8年后复发后已成功治疗的T细胞原代中枢神经系统

• 发表时间: 2011
• 作者: 真家紘一郎;鈴川和己;坂本竜弘;福田匡芳;日下部学;鈴木幸恵;栗田尚樹;錦井秀和;横山泰久;小原直;大越靖;長谷川雄一;千葉滋
• 通讯作者: 千葉滋