The analysis of the hypoxia-induced regulation mechanism in erythropetin gene expression and erythrocytosis

最近，有研究表明，骨髓基质细胞中可能存在表达Nestin基因的神经干细胞标记物，这些细胞在造血过程中发挥着重要作用。因此，我们杂交了RBP-J条件基因敲除小鼠。RBP-J是Notch信号的下游基因。和NestinCre小鼠，并分析了表达Nestin的细胞特异性RBP-J信号被敲除的小鼠的造血功能。基因敲除小鼠在胚胎期出现严重贫血，其红细胞分化受阻，促红细胞生成素水平显著降低。胎肝的促红细胞生成素产生细胞产生巢蛋白，提示胎肝基质细胞在造血分化中可能起重要作用。

Recently, the possibility has been suggested that there are bone marrow stromal cells expressing Nestin genes which are neural stem cell markers and that those cells play an important role in hematopoiesis. Therefore, we crossbred RBP-J conditional-knockout mice. RBP-J are downstream genes of Notch signaling. and NestinCre mice and analyzed the hematopoiesis of those mice with which Nestin-expressing-cell-specific RBP-J signaling was knocked out. The fetuses of the knockout mice suffered from severe anemia during embryonic stage, the differentiation of their erythroblasts was impaired and the onset of erythropoietin was significantly lowered. The erythropoietin productive cells of fetal livers developed Nestin and it was suggested that stromal cells of fetal livers may be important to hematopoietic differentiation.