Analysis of epigenetic aberration in plasma cell dyscrasia

我们分析了使用或不使用去甲基化剂处理的MM细胞的基因表达谱，以筛选DNA甲基化沉默的肿瘤相关基因。我们接下来研究了逆转录转座子DNA低甲基化对染色体畸变的影响。染色体缺失的MM患者的LINE-1甲基化水平明显低于没有缺失的患者，这表明LINE-1低甲基化与染色体不稳定性有关。我们正在进行的研究是使用高通量下一代测序仪破译MM细胞中的甲基组；并评估甲基组状态和基因组状态之间的关系。

We analyzed gene expression profile in MM cells treated with or without demethylating agent to screen for the tumor related genes silenced by DNA methylation. We next examined the effect of DNA hypomethylation in retrotransposon for chromosomal aberration. Patients in MM with chromosomal deletion showed significant lower LINE-1 methylation levels than patients without deletion suggesting that LINE-1 hypomethylation are associated with chromosomal instability. Our ongoing study is to decipher the methylome in MM cells using high-throughput next-generation sequencer ; and assess the association between the methylome status and the genome status.