Serum analysis of patients with Kawasaki disease using proteomic approaches

使用蛋白质组学方法对川崎病患者进行血清分析

• 批准号: 22790966
• 负责人: SHIMIZU Nobutaka
• 金额: $ 2.5万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22790966/
• 关键词: 川崎病; プロテオミクス; 血管炎; 自己抗体

We investigated the possibility that sera in patients with Kawasaki disease has unique antibody reacting to endothelial cells. We performed proteomic analysis using sera by 2D-DIGE(2 dimensional Fluorescence Difference Gel Electrophoresis), and found that patients have several unique antibodies which react to proteins expressed in endothelial cells. By quantitative analysis by ELISA assay, patients with Kawasaki disease have higher amount of one of the antibody. Generation of the auto-antibody may underlie the pathogenesis of Kawasaki disease

我们研究了川崎病患者血清中对内皮细胞有独特抗体反应的可能性。我们使用2D-DIGE（二维荧光差异凝胶电泳）对血清进行蛋白质组学分析，发现患者有几种独特的抗体可以对内皮细胞中表达的蛋白质起反应。ELISA法定量分析川崎病患者其中一种抗体含量较高。自身抗体的产生可能是川崎病发病机制的基础