A role of nuclear import activity on the proliferation of cutaneous diseases

核输入活动对皮肤疾病扩散的作用

• 批准号: 22791070
• 负责人: UMEGAKI Noriko
• 金额: $ 2.58万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22791070/
• 关键词: 核移行シグナル; karyopherinα2; TAP methods; mass spectrometry; MTSアッセイ; karyopherin α; 増殖; LC/MS/MS

Detection and analysis of target proteins imported by karyopherin alpha 2(KPNA2) in HaCaT cells using tandem affinity purification method and mass spectrometry to investigate the roles of KPNA2 in the cell growth. Immunohistochemical staining using anti-KPNA2 antibody was performed in the skin diseases including psoriasis and squamous cell carcinomas to demonstrate the relation ship between KPNA2 and keratinocyte proliferation.

利用串联亲和纯化技术和质谱技术检测和分析KPNA2在HaCaT细胞中导入的靶蛋白，探讨KPNA2在HaCaT细胞生长中的作用。采用抗KPNA2抗体对银屑病和鳞状细胞癌等皮肤病进行免疫组化染色，以证明KPNA2与角质形成细胞增殖的关系。

项目成果

Pregnancy and childbirth in three mothers with recessive dystrophic epidermolysis bullosa

三名隐性营养不良性大疱性表皮松解症母亲的妊娠和分娩

• 发表时间: 2012
• 期刊: Clinical and experimental dermatology
• 影响因子: 4.1
• 作者: Hanafusa T;Tamai K;Umegaki N;Yamaguchi Y;Fukuda S;Nishikawa Y;Yaegashi N;Okuyama R;McGrath JA;Katayama I
• 通讯作者: Katayama I

PDGFRalpha-positive cells in bone marrow are mobilized by high mobility group box 1 (HMGB1) to regenerate injured epithelia

骨髓中的 PDGFRα 阳性细胞被高迁移率族蛋白 1 (HMGB1) 动员以再生受损的上皮细胞

• 发表时间: 2011
• 期刊: Proc Natl Acad Sci
• 作者: Tamai K;Yamazaki T;Chino T;Ishii M;Otsuru S;Kikuchi Y;Iinuma S;Saga K;Nimura K;Shimbo T;Umegaki N;Katayama I;Miyazaki J;Takeda J;McGrath JA;Uitto J;Kaneda Y
• 通讯作者: Kaneda Y

PDGFR alpha-positive cells in bone marrow are mobilized by high mobility group box 1(HMGB1) to regenerate injured epithelia

骨髓中的 PDGFR α 阳性细胞被高迁移率族蛋白 1 (HMGB1) 动员以再生受损的上皮细胞

• 发表时间: 2011
• 期刊: Proc Natl Acad Sci
• 作者: Tamai K;Yamazaki T;Chino T;Ishii M;Otsuru S;Kikuchi Y;Iinuma S;Saga K;Nimura K;Shimbo T;Umegaki N;Katayama I;Miyazaki J;Takeda J;McGrath JA;Uitto J;Kaneda Y
• 通讯作者: Kaneda Y

Vorner type palmoplantar keratoderma: novel KRT9 mutation associated with knuckle pad-like lesion and recurrent mutation causing digital mutilation.

Vorner 型掌跖角化病：与指节垫样病变相关的新型 KRT9 突变和导致指残的复发突变。

• 发表时间: 2011
• 期刊: Br J Dermatol
• 作者: Umegaki N;Nakano H;Tamai K;Mitsuhashi Y;Akasaka E;Sawamura D;Katayama I
• 通讯作者: Katayama I

A case of giant squamous cell carcinoma of the buttock possibly arose from syringocystadenoma and invaded to the rectum.

• DOI: 10.1155/2011/213406
• 发表时间: 2011
• 期刊: Journal of skin cancer
• 影响因子: 1.1
• 作者: Nishioka M;Tanemura A;Yamanaka T;Umegaki N;Tani M;Katayama I;Takemasa I;Sekimoto M;Tomita K;Tamai N
• 通讯作者: Tamai N