Induction of tolerance to small intestine allografts by adoptive transfer of in vitro generating regulatory T-cells

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We demonstrate the in vitro generation of regulatory T cells(RA-Treg) in the presence of IL-2, TGF beta and all trans retinoic acid(RA) to acquire a gut-homing ability and more potent at suppressing T-cell activation in vitro. We also demonstrate that RA-Treg injected into Naive mice, intestinal ischemia/reperfusion injury mice, syngeneic intestine transplanted mice, allogeneic intestine transplanted mice respectively can reach recipient mesenteric nodes. More increased accumulation of RA-Treg was observed in I/R injury mice, syngeneic transplanted mice, allogeneic transplanted mice than that in naive mice demonstrating the role of I/R injury for accumulation Tregs in this model.