Role of the FOXF2 gene in the cancer activated fibroblasts that support proliferation of colon cancer cells

FOXF2 基因在支持结肠癌细胞增殖的癌症激活成纤维细胞中的作用

• 批准号: 22791272
• 负责人: SULTANA Nishat
• 金额: $ 2.58万
• 依托单位: Hamamatsu University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22791272/
• 关键词: 癌付随線維芽細胞; 大腸癌; Foxf2遺伝子; 増殖; 腫瘍微小環境; 癌細胞増殖; FOXF2; 上皮-間葉相互作用; 転移能; Wnt5a; Wint

When colon cancer cells HT29 were transplanted with intestinal embryonic fibroblasts(IEFs) from the Foxf2-/-and wild-type mice, the efficiency and tumor size were highest in cotranslantation with the wild-type IEFs, higher in that with the Foxf2-/-IEFs compared to that without fibroblasts. I isolated RNas from IEFs from the Foxf2-/-and wild-type mice and applied to DNA microarray analysis. I found 23 genes were significantly higher in the wild-type compared to the Foxf2-/-mice. I chose one gene named Wnt5a and demonstrated that the Wnt5a-expressing fibroblasts increased the efficiency and tumor size of HT29 cell transplantation.

当结肠癌细胞HT29与来自Foxf2-/-和野生型小鼠的肠胚胎成纤维细胞(IEF)一起移植时，与野生型IEF共移植的效率和肿瘤大小最高，与没有成纤维细胞的相比，与Foxf2-/-IEF共移植的效率和肿瘤大小更高。我从 Foxf2-/- 和野生型小鼠的 IEF 中分离出 RNA，并将其应用于 DNA 微阵列分析。我发现野生型小鼠中有 23 个基因显着高于 Foxf2-/- 小鼠。我选择了一个名为 Wnt5a 的基因，并证明表达 Wnt5a 的成纤维细胞增加了 HT29 细胞移植的效率和肿瘤大小。

项目成果

Liver tumor formation by a mutant retinoblastoma protein in the transgenic mice is caused by an up-regulation of c-Myc target genes

转基因小鼠中突变视网膜母细胞瘤蛋白形成的肝脏肿瘤是由 c-Myc 靶基因上调引起的

• 发表时间: 2012
• 期刊: Biochem Biophys Res Commun
• 作者: Wang B;Hikosaka K;Sultana N;Sharkar MTK;Noritake H;Kimura W;Wu Y-X;Kobayashi Y;Uezato T;Miura N
• 通讯作者: Miura N

Irxl1 mutant mice show reduced tendon differentiation and no pattening defects in musculoskletal system development.

Irxl1 突变小鼠表现出肌腱分化减少，并且在肌肉骨骼系统发育中没有模式缺陷。

• 发表时间: 2011
• 期刊: Genesis
• 影响因子: 1.5
• 作者: Kimura;W.;et al.
• 通讯作者: et al.

Irx3 determines expression of fast and slow connexins in the ventricular conduction system of the mouse heart

Irx3 决定小鼠心脏心室传导系统中快速和慢速连接蛋白的表达

• 发表时间: 2011
• 作者: Sultana N;他
• 通讯作者: 他

The mouse forkhead gene Foxp2 modulates expression of the lung genes.

• DOI: 10.1016/j.lfs.2010.05.009
• 发表时间: 2010-07
• 期刊: Life sciences
• 影响因子: 6.1
• 作者: Zhi Yang;Keisuke Hikosaka;M. Sharkar;T. Tamakoshi;Abhishek Chandra;Bo Wang-;Tatsuo Itakura;Xiaodong Xue;T. Uezato;Wataru Kimura;N. Miura

Generation of the Tbxl-AmCyan transgenic mice and its application.

Tbxl-AmCyan转基因小鼠的产生及其应用。

• 发表时间: 2011
• 作者: Miura;N.;et al.
• 通讯作者: et al.