Establishment of a tailor-made chemotherapy of esophageal cancer, based on chemosensitivity test combining search of molecular reaction.

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An accurate culture drug sensitivity test may redound to chemotherapy for cancer. In this study, we attempted to establish a new predicting system for drug sensitivity by monitoring molecular signaling pathways in esophageal cancer(ESC) cell line. We firstly evaluated cell signaling affected by CDDP, a key drug for ESC chemotherapy in ESC cell lines. However, it was difficult to interpret the behavior of examined molecules, because multiple pathways were intricately involved in CDDP stimulation. As more simple analysis object, we verified to monitor the detailed cell signaling affected by cetuximab in colorectal cancer cell line HT29 at the protein level using reversephase protain lysate microarrays, with the aim of establishing an accurate analysis, in addition to the conventional Western blotting. In EGF pathway, we were able to quantitative monitor by variation of dephosphorylated protein with time.