Intrapleural administration of gelatin-embedded sustained-release basic fibroblast growth factor for the regeneration of emphysematous lungs in rats

动物实验表明，明胶包埋缓释碱性成纤维细胞生长因子（SRBF）经气道和动脉内给药可刺激肺气肿肺的再生，但这些给药途径也可能造成伤害。本研究探讨了SRBF胸腔内注射的有效性。用猪弹性蛋白酶诱导大鼠肺气肿。SRBF经左胸膜腔给药。将大鼠分为给予SRBF组（FGF+组）和不给予SRBF组（FGF-组）。14 d后处死动物，通过组织学检查评价结果。在FGF+组中，治疗侧肺泡间隔的平均线性截距值明显短于未治疗侧（65.1 7.0 μm vs 114.4±7.5 μm, P = 0.0005）。在FGF-组中，治疗侧和未治疗侧的平均线性截距值无显著差异。胸膜内注射SRBF因子诱导弹性酶诱导肺气肿大鼠肺再生。

Intra-airway and intra-arterial administration of gelatin-embedded sustained-release basic fibroblast growth factor (SRBF) have been shown to stimulate regeneration of emphysematous lungs in animal experiments, but these routes of administration may also cause harm. This study investigated the effectiveness of intrapleural administration of SRBF. Pulmonary emphysema was induced in Sprague-Dawley rats using porcine elastase. SRBF was administered via the left pleural cavity. The rats were divided into a group that received SRBF (FGF+ group), and a group that did not (FGF- group). Animals were sacrificed after 14 days, and the results were evaluated by histological examination. In the FGF+ group, the mean linear intercept value of the alveolar septa was significantly shorter on the treated side than on the untreated side (65.1 7.0 μm vs 114.4 ± 7.5 μm, P = .0005). There was no significant difference in the mean linear intercept value between the treated and untreated sides in the FGF- group. Intrapleural administration of SRBF factor induced lung regeneration in rats with elastase-induced pulmonary emphysema.