OASIS, a CREB/ATF-family transcription factor, modulate transcription of C6ST1 gene and chondroitin sulfation

在中枢神经系统，内质网应激涉及广泛的疾病，包括缺血性损伤和神经退行性疾病。损伤后皮层中OASIS mRNA和蛋白水平均升高，且与星形胶质细胞的表达密切相关。反应性星形胶质细胞是胶质瘢痕的主要细胞成分，它会缠绕病变部位并产生抗再生分子，如硫酸软骨素蛋白多糖（CSPGs）。我们用OASIS敲除小鼠分析了cs -糖胺聚糖合成酶的mRNA水平。野生型小鼠皮质末梢损伤时，骨软素6-硫转移酶1（C6ST1）表达增加，而OASIS敲除小鼠无此表达。此外，OASIS结合到第一个内含子区域，促进C6ST1的转录。这些结果表明OASIS可能通过星形胶质细胞中C6ST1的转录参与CSPG的产生。

In the central nerve system, ER stress is implicated in a wide range of disorders including ischemic injury and neurodegenerative disorder. Both mRNA and protein levels of OASIS were elevated in the injured cortex and correlated closely with those of reactive astrocyte. Reactive astrocyte, which constitute a major cellular component of glial scar, enmesh the lesion site and produce anti-regenerative molecules such as chondroitin sulphate proteoglycans(CSPGs). We have analyzed the mRNA levels of the CS-glycosaminoglycan synthesizing enzymes using OASIS knockout mice. Expression of chondroitin 6-sulfotransferase 1(C6ST1) is increased in cortical stub injury in wild type mice, but is not in OASIS knock out mice. Furthermore, OASIS bound to the first intron region and promoted transcription of C6ST1.These results suggest that OASIS may be involved in CSPG production through the transcription of C6ST1 in astrocytes.