N-Acetylglucosamine 6-O-Sulfotransferase-1 is crucialenzyme for 5D4-reactive keratin sulfate biosynthesis which elicits expression after spinal cord injury.

N-乙酰葡糖胺 6-O-磺基转移酶-1 是 5D4 反应性硫酸角蛋白生物合成的关键酶，可在脊髓损伤后引发表达。

• 批准号: 22791371
• 负责人: ANDO Kei
• 金额: $ 1.75万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22791371/
• 关键词: GlcNAc6ST-1; 脊髄損傷; 脊髄腫瘍; KSPG; 5D4; KSGal6ST-1

：5D4-reactive KSPGs were decrease as aging in wild type mice CNS, and were loss of expression in GlcNAc6ST-1-/- mice. On the other hand, BCD4-reactive KSPGs were increased as aging in wild type and GlcNAc6ST- 1-/- mice CNS. The KS enzyme b3GlcNAcT-7 was also significantly down-regulated not only GlcNAc6ST-1 in CNS of GlcNAc6ST-1-/- mice compared to that of wild type mice. 5D4-reactive KSPGs were decreased for cell lysates of GlcNAc6ST-1 siRNA transfected BV2, although BCD4-reactive KSPGs were not decreased. The enzyme b3GlcNAcT-7 was significantly downregulated not only GlcNAc6ST-1 same as in vivo study. The enzyme significantly down-regulated was only b3GlcNAcT-7. If the ablation of 5D4-reactive KS except for BCD4-reactive KS is possible, better functional recovery may be obtained. Ourdata suggested that GlcNAc6ST-1 was important enzyme for 5D4-reactive KSPGs biosynthesis, b3GlcNAcT-7 for both 5D4 and BCD4-reactive KSPGs biosynthesis. The data was supported with in vitro study used transfected microglia cell line. Interestingly, GlcNAc6ST-1 may regulate b3GclNAcT-7 by decrease of own expression. GlcNAc6ST-1 specific down-regulation treatment may be the key in CNS injury.

：5D4-反应性KSPG在野生型小鼠CNS中随着年龄的增长而减少，并且在GlcNAc 6ST-1-/-小鼠中失去表达。另一方面，在野生型和GlcNAc 6ST- 1-/-小鼠CNS中，BCD 4反应性KSPG随着年龄的增长而增加。与野生型小鼠相比，不仅GlcNAc 6ST-1-/-小鼠CNS中的GlcNAc 6ST-1，KS酶b3 GlcNAcT-7也显著下调。对于GlcNAc 6ST-1 siRNA转染的BV 2的细胞裂解物，5D4反应性KSPG降低，尽管BCD 4反应性KSPG没有降低。与体内研究相同，不仅GlcNAc 6ST-1，b3 GlcNAcT-7也显著下调。显著下调的酶仅为b3 GlcNAcT-7。如果除BCD 4反应性KS外的5D 4反应性KS的消融是可能的，则可以获得更好的功能恢复。结果表明，GlcNAc 6ST-1是5D4反应性KSPGs生物合成的重要酶，b3 GlcNAcT-7是5D4和BCD 4反应性KSPGs生物合成的重要酶。使用转染的小胶质细胞系的体外研究支持该数据。有趣的是，GlcNAc 6ST-1可能通过降低自身表达来调节b3 GclNAcT-7。GlcNAc 6ST-1特异性下调治疗可能是CNS损伤的关键。

项目成果

Examination of the influence of ossification of the anterior longitudinal ligament on symptom progression and surgical outcome of ossification of the thoracic ligamentum flavum: a multicenter study

检查前纵韧带骨化对胸椎黄韧带骨化症状进展和手术结果的影响：一项多中心研究

• 发表时间: 2012
• 期刊: J Neurosurg Spine
• 作者: Kei Ando;Imagama S;Wakao N;Hirano K;Tauchi R;Muramoto A;Kato F;Yukawa Y;Kawakami N;Sato K;Matsubara Y;Kanemura T;Matsuyama Y;Ishiguro N
• 通讯作者: Ishiguro N

N-Acetylglucosamine 6-O-Sulfotransferase-1 is Crucial Enzyme for 5D4-Reactive-Keratan Sulfate Biosynthesis which Elicits Expression after Spinal Cord Injury

N-乙酰葡糖胺 6-O-磺基转移酶-1 是 5D4-反应性硫酸角质素生物合成的关键酶，可在脊髓损伤后引发表达

• 发表时间: 2011
• 作者: 関 庄二;淺沼由美子;舛田浩一;川口善治;淺沼邦洋;木村友厚;Kei Ando;安藤圭
• 通讯作者: 安藤圭

Mutual and hierarchical regulation of expression of keratan sulfate biosynthesis enzymes

硫酸角质素生物合成酶表达的相互和分层调节

• 发表时间: 2011
• 期刊: Glycobiology
• 影响因子: 4.3
• 作者: Kei Ando;Imagama S;Wakao N;Hirano K;Tauchi R;Muramoto A;Kato F;Yukawa Y;Kawakami N;Sato K;Matsubara Y;Kanemura T;Matsuyama Y;Ishiguro N;河本旭哉;安藤圭
• 通讯作者: 安藤圭

N-Acetylglucosamine 6-O-Sulfotransferase-1 is crucial enzyme for 5D4-reactive keratin sulfate biosynthesis which elicits expression after spinal cord injury

N-乙酰葡萄糖胺 6-O-磺基转移酶-1 是 5D4 反应性硫酸角蛋白生物合成的关键酶，可在脊髓损伤后引发表达

• 发表时间: 2011
• 作者: 関 庄二;淺沼由美子;舛田浩一;川口善治;淺沼邦洋;木村友厚;Kei Ando
• 通讯作者: Kei Ando

N-Acetylglucosamine 6-O-Sulfotransferase-1-Deficient Mice Show Better Functional Recovery after Spinal Cord Injury

• DOI: 10.1523/jneurosci.2570-09.2010
• 发表时间: 2010-04-28
• 期刊: JOURNAL OF NEUROSCIENCE
• 影响因子: 5.3
• 作者: Ito, Zenya;Sakamoto, Kazuma;Kadomatsu, Kenji
• 通讯作者: Kadomatsu, Kenji