Cellular and molecular biological analysis of the impact of drugs used during periorerative period on cellular oxygen metabolism

围手术期药物对细胞氧代谢影响的细胞和分子生物学分析

• 批准号: 22791428
• 负责人: TAKABUCHI Satoshi
• 金额: $ 2.58万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22791428/
• 关键词: 薬理学; 麻酔薬; 低酸素応答; 細胞生物学; 分子生物学; 麻酔科学

We demonstrated that Glucose-stimulated insulin secretion(GSIS) elicited by 400 mg/dL glucose was inhibited by treatment with isoflurane and sevoflurane. Taken together experimental evidence that glibenclamide-stimulated insulin secretion was not affected by either isoflurane or sevoflurane and that treatment with isoflurane or sevoflurane significantly suppress glucose-induced intracellular ATP elevation, it is suggested that isoflurane and sevoflurane affect mitochondria ETC and suppresses GSIS. Our finding may delineate the molecular mechanism behind the glucose tolerance established by volatile anesthetics.

我们证明了400 mg/dL葡萄糖引起的葡萄糖刺激胰岛素分泌（GSIS）被异氟醚和七氟醚处理抑制。综合实验证据，格列本脲刺激的胰岛素分泌不受异氟醚和七氟醚的影响，异氟醚和七氟醚治疗可显著抑制葡萄糖诱导的细胞内ATP升高，提示异氟醚和七氟醚可影响线粒体ETC并抑制GSIS。我们的发现可能描述了挥发性麻醉药建立葡萄糖耐量的分子机制。

项目成果

The intravenous anesthetic propofol inhibits lipopolysaccharide-induced hypoxia-inducible factor 1 activation and suppresses the glucose metabolism in macrophages

• DOI: 10.1007/s00540-009-0829-1
• 发表时间: 2010-02-01
• 期刊: JOURNAL OF ANESTHESIA
• 影响因子: 2.8
• 作者: Tanaka, Tomoharu;Takabuchi, Satoshi;Hirota, Kiichi
• 通讯作者: Hirota, Kiichi

Differential role of prostaglandin E-type receptors in activation of hypoxia-inducible factor1 by prostaglandin E1 in vascular-derived cells under non-hypoxic conditions

非缺氧条件下前列腺素E型受体在前列腺素E1激活血管源性细胞缺氧诱导因子1中的差异作用

• 发表时间: 2011
• 作者: Kengo Suzuki;Satoshi Takabuchi;Hiroki Daijo;Shinichi Kai;Tomonori Matsuyama;Shin Kurosawa;Kazuhiko Fukuda;Kiichi Hirota
• 通讯作者: Kiichi Hirota

Hydrogen sulfide inhibits hypoxia-induced HIF-1 activation in a mitochondria-dependent manner

硫化氢以线粒体依赖性方式抑制缺氧诱导的 HIF-1 激活

• 发表时间: 2011
• 作者: Shinichi Kai;Tomoharu Tanaka;Hiroki Daijo;Shun Kishimoto;Kengo Suzuki;Satoshi Takabuchi;Takuhiko Wakamatsu;Kazuhiko Fukuda;Kiichi Hirota
• 通讯作者: Kiichi Hirota