Evaluation of completeness using different diameters and types of suture materials for chronic constriction injury model in rat

我们使用不同直径和类型的缝合材料来评估Bennett方法的完整性。在本研究中，(1)损伤腿的手指变形和疼痛相关行为，(2)自动行为分析（饲养次数、饲养长度和总步行距离）和(3)坐骨神经的组织学表现进行了检查。结果：坐骨神经收缩后4周，1-0丝线组5例中有4例，4-0丝线组5例中有1例，4-0尼龙线组7例中有4例出现手指变形及疼痛相关行为。坐骨神经的组织学改变以1-0丝线> - 4-0尼龙缝线> - 4-0丝线排列。讨论：我们发现1-0丝是大鼠慢性收缩损伤模型（Bennett模型）的最佳缝合材料。

We evaluated the completeness of Bennett method using different diameters and types of suture materials. In this study, (1) digit transformation of injured leg and the pain-related behavior, (2)automatic behavior analysis (the number of rearing, length of rearing and total walking distance) and (3) histologic findings of sciatic nerve were examined.Results: four weeks after constriction of sciatic nerve, digit transformation and the pain-related behavior was showed with 4 of 5 in 1-0 silk thread group, 1 of 5 in 4-0 silk thread group, 4 of 7 in 4-0 nylon sutures group. Histological changes appeared in sciatic nerve was frequent in order of 1-0 silk thread> 4-0 nylon suture> 4-0 silk thread.Discussion: We found that the 1-0 silk is the best suture material for chronic constriction injury model (Bennett model) in rat.