Regeneration of cavernous nerve after transplantation of CD 133+ cells derived from human peripheral blood and placement of biodegradable gel sponge sheet.

移植来自人外周血的 CD 133 细胞并放置可生物降解的凝胶海绵片后海绵体神经的再生。

• 批准号: 22791475
• 负责人: SHOGO Inoue
• 金额: $ 2.66万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22791475/
• 关键词: 前立腺癌; 神経再生; 陰茎海綿体神経; アルギン酸ゲルシート; CD133陽性細胞; 前立腺; 陰茎海綿体神

The aim of this study was to develop an easier nerve-regenerating technique without sutures by applying an alginate gel sponge sheet and investigate acceleration of axonal regeneration induced by the transplantation of the human peripheral blood-derived CD133+ cells into a rat cavernous nerve defect model. The intracavernous pressure(ICP) response elicited by elemental stimulation of the major pelvic ganglion(MPG) was evaluated. The MPG was exposed, and a needle was into the corpus cavernosum and the femoral artery, respectively, to monitor the ICP and mean arterial pressure(MAP). There is no significant difference in ICP/ MAP ratio between the alginate sheet group and the cavernous nerve dissection group(p=0. 74). In contrast, ICP/ MAP ratio is significantly higher in the CD133+ cells transplantation group than the cavernous nerve dissection group(p=0. 01).

本研究的目的是通过应用藻酸盐凝胶海绵片开发一种更容易的无缝合神经再生技术，并研究将人外周血来源的CD 133+细胞移植到大鼠海绵状神经缺损模型中诱导轴突再生的加速作用。评价了由骨盆大神经节（MPG）的元素刺激引起的海绵体内压力（ICP）反应。暴露MPG，将针头分别插入海绵体和股动脉，监测ICP和平均动脉压（MAP）。藻酸盐片材组和海绵体神经剥离组之间的ICP/ MAP比率没有显著差异（p=0. 001）。74）。相反，在CD 133+细胞移植组中ICP/ MAP比显著高于海绵体神经剥离组（p=0. 001）。01）。

项目成果

ヒト骨髄由来CD133陽性細胞を用いたラット陰茎海綿体神経の再生

使用人骨髓来源的 CD133 阳性细胞再生大鼠阴茎海绵体神经

• 发表时间: 2012
• 作者: 宮本克利;井上省吾;池田健一郎;稗田圭介;正路晃一;小林加直;梶原充;亭島淳;松原昭郎
• 通讯作者: 松原昭郎