Regeneration of cavernous nerve after transplantation of CD 133+ cells derived from human peripheral blood and placement of biodegradable gel sponge sheet.

本研究的目的是通过应用海藻酸盐凝胶海绵片开发一种更容易的无缝合神经再生技术，并研究将人外周血来源的CD133+细胞移植到大鼠海绵状神经缺损模型中对轴突再生的加速作用。评估元素刺激盆腔大神经节(MPG)引起的海绵体内压(ICP)反应。显露MPG，分别将针刺入海绵体和股动脉，监测颅内压和平均动脉压。海藻酸盐膜组和海绵体神经剥离组之间的颅内压/平均动脉压无显著差异(p=0。74)。相比之下，CD133+细胞移植组的颅内压/平均动脉压显著高于海绵体神经剥离组(p=0。01)。

The aim of this study was to develop an easier nerve-regenerating technique without sutures by applying an alginate gel sponge sheet and investigate acceleration of axonal regeneration induced by the transplantation of the human peripheral blood-derived CD133+ cells into a rat cavernous nerve defect model. The intracavernous pressure(ICP) response elicited by elemental stimulation of the major pelvic ganglion(MPG) was evaluated. The MPG was exposed, and a needle was into the corpus cavernosum and the femoral artery, respectively, to monitor the ICP and mean arterial pressure(MAP). There is no significant difference in ICP/ MAP ratio between the alginate sheet group and the cavernous nerve dissection group(p=0. 74). In contrast, ICP/ MAP ratio is significantly higher in the CD133+ cells transplantation group than the cavernous nerve dissection group(p=0. 01).