Genetic chemosensitivity test with novel drug-resistance genes in epithelial ovarian cancer

我们建立了两个卵巢癌细胞系，它们显示出对紫杉醇/顺铂的耐药性。通过基因芯片分析，筛选出8个候选基因（ABCB 1、ABCG 2、IL 6、IL 8、DR 5、p21、KISS 1、MMP 1），这些基因在耐药细胞中的表达水平明显高于亲本细胞。对这些卵巢癌细胞系中8个基因的定量分析结果支持这一结论。获得知情同意后，提供了13例卵巢癌临床样本和信息，可用于数据库。对13例临床标本中这8个基因的定量分析表明，耐药组的表达水平高于敏感组。

We established two ovarian cancer cell lines which showed drug resistance for Paclitaxel/Cisplatin from their parental cell lines. The cDNA microarray analysis of these parental and resistant cell proposed eight candidate genes(ABCB1, ABCG2, IL6, IL8, DR5, p21, KISS1, MMP1) for the novel chemo-sensitivity test, which levels in resistant cell were significantly higher than in parental cell. The result of quantitative analysis of the eight genes in these ovarian cancer cell lines supported this consequence. Gaining informed consent, 13 ovarian cancer clinical samples and information were provided, which is available for database. The quantitative analysis of these eight genes in these 13 clinical samples revealed that the levels of resistant group were higher than sensitive group.