Molecular biological analysis of otitis media with effusion

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Nontypable Haemophilus Influenzae(NTHi) is one of the causative pathogen of otitis media with effusion and form biofilm. We determined antimicrobial agents susceptibility of NTHi from clinical isolates in biofilm with the new minimal biofilm eradication concentration(MBEC) method. In addition, we have assessed the effect of antimicrobial exposure timing.There were large differences in susceptibility of NTHi biofilm between antimicrobial agents. Beta-lactams had little effect on eradication bacteria in biofilm and biofilm volume. However, macrolides were comparatively effective. Biofilm was inhibited by antimicrobial agents in the early stage of biofilm. However, antimicrobials did not decrease mature biofilm.