The effect of potassium and calcium on wood formation and xylem/phloem physology

钾和钙对木材形成和木质部/韧皮部生理的影响

• 批准号: 5398233
• 负责人: Professor Dr. Jörg Fromm
• 金额: --
• 依托单位: Abteilung Holzbiologie
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2003
• 资助国家: 德国
• 起止时间: 2002-12-31 至 2009-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5398233?language=en
• 关键词: effect; potassium; calcium; wood; formation

Ions play a fundamental role in the physiology of cambial growth. To gain better knowledge about the role of K, Ca and P in wood formation, we intend to focus on plants grown under different K, Ca and P supply as well as on transgenic plants with modified ion transporter expression produced by P5 and/or P3. Two approaches will be applied on all differently treated plants in this project. First, structural and ultrastructural analysis of stem tissues (phloem, cambium, xylem) will be carried out throughout all seasons by image analysis and high resolution TEM. In order to correlate structural changes to biochemical variations, a second approach deals with the following analysis in all tissues: Seasonal changes of K, Ca and P will be measured by EDXA, whereas K and Ca will also be determined quantitatively by atomic absorption spectrometry. By generating antibodies against different potassium transporters we further will show their distribution in poplar stem tissues throughout all seasons by fluorescence and transmission electron microscopy. In order to correlate changes in ion content to sugar concentrations, seasonal variations of different sugars as well as starch will be determined enzymatically. To measure changes in the chemical composition of cell walls, FTIR-spectroscopy will be used to quantitatively detect a range of functional groups in the cell wall.

离子在形成层生长的生理过程中起着重要的作用。为了更好地了解K，Ca和P在木材形成中的作用，我们打算专注于不同的K，Ca和P供应下生长的植物，以及P5和/或P3产生的修饰的离子转运蛋白表达的转基因植物。在本项目中，两种方法将应用于所有不同处理的植物。首先，将通过图像分析和高分辨率TEM在整个季节进行茎组织（韧皮部、形成层、木质部）的结构和超微结构分析。为了将结构变化与生物化学变化相关联，第二种方法涉及所有组织中的以下分析：K，Ca和P的季节性变化将通过EDXA测量，而K和Ca也将通过原子吸收光谱法定量测定。通过产生针对不同钾转运蛋白的抗体，我们将进一步通过荧光和透射电子显微镜显示它们在所有季节的白杨茎组织中的分布。为了将离子含量的变化与糖浓度相关联，将通过酶法确定不同糖以及淀粉的季节性变化。为了测量细胞壁化学组成的变化，FTIR光谱将用于定量检测细胞壁中的一系列官能团。