The mechanism of non-steroidal anti-inflammatory drugs on osteoclast differentiation and activation.

非甾体抗炎药影响破骨细胞分化和活化的机制。

• 批准号: 22791800
• 负责人: KARAKAWA Akiko
• 金额: $ 2.5万
• 依托单位: Showa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22791800/
• 关键词: 歯科薬理学; 破骨細胞; NSAIDs; COX; 歯学; osteoclast; cyclooxygenase

Non-steroidal anti-inflammatory drugs(NSAIDs) have regulatory effect on bone remodeling. The efficacy of NSAIDs on bone remodeling is controversial. In clinics, NSAIDs progress bone repair as their inhibitory effect of inflammatory factors ; in contrast, NSAIDs delay bone fracture healing in animal models. The aim of this study was to elucidate the mechanism of NSAIDs on osteoclast differentiation and bone resorptive activity.Cyclloxygenase(COX)-1 and-2 transcripts were appeared in early stage of osteoclast differentiation(day3). COX-2 increased on mature osteoclasts and lipopolysaccharide stimulated inflammatory mature osteoclasts. Level of COX-2 transcript has efficacy on extracellular signal-regulated kinase appearance. These results showed that COX-1 and-2 involved in osteoclasts have regulatory effect on osteoclasts differentiation and bone resorptive activity.

非甾体类抗炎药（NSAIDs）对骨重塑具有调节作用。 NSAIDs 对骨重塑的功效存在争议。临床上，NSAIDs通过抑制炎症因子的作用促进骨修复；相比之下，非甾体抗炎药会延迟动物模型中的骨折愈合。本研究旨在阐明NSAIDs对破骨细胞分化和骨吸收活性的影响机制。环加氧酶(COX)-1和-2转录本在破骨细胞分化早期(第3天)出现。 COX-2 在成熟破骨细胞和脂多糖刺激的炎症成熟破骨细胞上增加。 COX-2 转录物水平对细胞外信号调节激酶的出现有影响。这些结果表明参与破骨细胞的COX-1和-2对破骨细胞的分化和骨吸收活性具有调节作用。

项目成果

Pam3CSK4, a TLR2 agonist, induces in vitro osteoclastogenesis RANKL-independently and resorbs alveolar bone in rats

Pam3CSK4 是一种 TLR2 激动剂，可独立诱导体外破骨细胞生成 RANKL 并吸收大鼠牙槽骨

• 发表时间: 2011
• 作者: Yano A;Suzuki K;Morohashi T;Arimoto T;Karakawa A;Igarashi T;Shinoda H;Yamamoto M;Yamada S
• 通讯作者: Yamada S

ホームページ昭和大学研究成果データベース

主页 昭和大学研究成果数据库

TLR2アゴニストであるPam3CSK4による破骨細胞形成とラット上顎口蓋骨骨吸収作用について

TLR2激动剂Pam3CSK4对破骨细胞形成和大鼠上颌骨骨吸收的影响

• 发表时间: 2011
• 作者: Yano A;Suzuki K;Morohashi T;Arimoto T;Karakawa A;Igarashi T;Shinoda H;Yamamoto M;Yamada S;矢野亜希子,鈴木恵子,諸橋富夫,有本隆文,唐川亜希子,五十嵐武,山本松男,山田庄司
• 通讯作者: 矢野亜希子,鈴木恵子,諸橋富夫,有本隆文,唐川亜希子,五十嵐武,山本松男,山田庄司

Effects of tetracycline hydrochloride on measurements with the laser fluorescence device DIAGNOdent: in vitro and in vivo studies

盐酸四环素对激光荧光装置 DIAGNOdent 测量的影响：体外和体内研究

• 发表时间: 2012
• 期刊: Lasers in Medical Science
• 影响因子: 2.1
• 作者: Eriko Nagaoka;T. Morohashi;J. Kinoshita;A. Karakawa;N. Sakai;S. Yamada;M. Inoue
• 通讯作者: M. Inoue

Non-steroidal Anti-inflammatory Drugs Inhibit Osteoclast Activation by Inhibiting Nuclear Translocation of NFκB.

非甾体抗炎药通过抑制 NFκB 核转位来抑制破骨细胞活化。

• 发表时间: 2010
• 作者: Akiko Karakawa;Hitoshi Amano;Keiko Suzuki;Shoji Yamada
• 通讯作者: Shoji Yamada