Development and clinical evaluation of soft tissue regeneration based on mechanobiology

基于力学生物学的软组织再生技术的发展及临床评价

• 批准号: 22791897
• 负责人: MASAKI Chihiro
• 金额: $ 2.58万
• 依托单位: Kyushu Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22791897/
• 关键词: 歯学; メカノバイオロジー; 再生医学; 細胞・組織; シグナル伝達; メカノセルバイオロジー

We focused on the effects of LIPUS on GE1 cells in this study. GE1 were exposed to LIPUS for 15 min at 3 MHz frequency and 40 mW/cm2 power. Total RNA was extracted after LIPUS exposure and analyzed by quantitative PCR to detect CCN2/CTGF, and total protein from each sample after LIPUS exposure was immunoblotted. CCN2/CTGF mRNA levels were significantly greater at 0 and 15 min after LIPUS exposure, compared with the control. Western blotting analysis showed intense staining of CCN2/CTGF for 60 min after LIPUS exposure. Phosphorylation of MAPK was up-regulated at 30 min after LIPUS exposure. Our findings demonstrated that LIPUS exposure increases CCN2/CTGF via the MAPK signaling pathway.

本研究重点关注 LIPUS 对 GE1 细胞的影响。 GE1 在 3 MHz 频率和 40 mW/cm2 功率下暴露于 LIPUS 15 分钟。 LIPUS 暴露后提取总 RNA，并通过定量 PCR 进行分析以检测 CCN2/CTGF，并对 LIPUS 暴露后每个样品的总蛋白进行免疫印迹。与对照相比，在 LIPUS 暴露后 0 和 15 分钟，CCN2/CTGF mRNA 水平显着升高。蛋白质印迹分析显示，LIPUS 暴露后 60 分钟，CCN2/CTGF 出现强烈染色。 LIPUS 暴露后 30 分钟，MAPK 磷酸化上调。我们的研究结果表明，LIPUS 暴露通过 MAPK 信号通路增加 CCN2/CTGF。

项目成果

The Effects of Low-Intensity Pulsed Ultrasound Exposure on Gingival Cells

低强度脉冲超声波照射对牙龈细胞的影响

• 发表时间: 2011
• 期刊: Journal of Periodontology
• 影响因子: 4.3
• 作者: R.Shiraishi;C.Masaki;A.Toshinaga;T.Okinaga;T.Nishihara;N.Yamanaka;T.Nakamoto;R.Hosokawa
• 通讯作者: R.Hosokawa

The effect of Low-Intensity Pulsed Ultrasound Exposure on Gingival Cells

低强度脉冲超声波照射对牙龈细胞的影响

• DOI: 10.1902/jop.2011.100627
• 发表时间: 2011
• 期刊: Journal of Periodontology
• 影响因子: 4.3
• 作者: R. Shiraishi;C. Masaki;A. Toshinaga;T. Okinaga;T. Nishihara;N. Yamanaka;T. Nakamoto;R. Hosokawa
• 通讯作者: R. Hosokawa