Bone regeneration by layered osteoblast cell sheets - rhBMP

本研究旨在通过成骨细胞片层植入促进骨缺损的愈合。首先，使用温度敏感培养皿获得大鼠成骨细胞（v-1细胞）的细胞片。然而，仅获得部分细胞片。此外，使用这些部分细胞片来制造分层的成骨细胞片是相当困难的。这些结果表明，是否成功获得细胞片取决于培养的细胞类型。

The aim of this study is to promote bone defect healing by layered osteoblast cell sheets implantation. First of all, temperature-responsive culture dishes were used to obtain cell sheets of rat osteoblast cells (v-1 cell). However, only partial cell sheets were obtained. Furthermore, it was quite difficult to make layered osteoblast cell sheets using these partial cell sheets. These results suggest that it depends on the type of cell cultured whether cell the sheet was successfully obtained or not.