Bone regeneration by layered osteoblast cell sheets - rhBMP

通过分层成骨细胞片层进行骨再生 - rhBMP

• 批准号: 22791945
• 负责人: HAYASHI Tatsuhide
• 金额: $ 2.5万
• 依托单位: Aichi Gakuin University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22791945/
• 关键词: 再生歯学; 骨再生; 骨芽細胞シート; 積層化; rhBMP-2; rhBMP

The aim of this study is to promote bone defect healing by layered osteoblast cell sheets implantation. First of all, temperature-responsive culture dishes were used to obtain cell sheets of rat osteoblast cells (v-1 cell). However, only partial cell sheets were obtained. Furthermore, it was quite difficult to make layered osteoblast cell sheets using these partial cell sheets. These results suggest that it depends on the type of cell cultured whether cell the sheet was successfully obtained or not.

本研究的目的是通过成骨细胞片层植入促进骨缺损的愈合。首先，采用温度敏感型培养皿获得大鼠成骨细胞(v-1细胞)的细胞片。然而，只获得了部分细胞片。此外，使用这些部分细胞片制作层状成骨细胞片是相当困难的。这些结果表明，细胞能否成功获得取决于培养的细胞类型。

项目成果

Promotion of rat calvarial defect healing by induced bone-like tissue.

诱导骨样组织促进大鼠颅骨缺损愈合。

• 发表时间: 2012
• 作者: T. Hayashi;S. Kobayashi;T. Asai;A. Mieki;H. Kataoka;Y. Uematsu;T. Kawai
• 通讯作者: T. Kawai

Bone-like tissue induced by rhBMPs in vitro has an ossification potential in vivo

rhBMPs 体外诱导的骨样组织在体内具有骨化潜力

• 发表时间: 2011
• 作者: Hayashi T;Asai T;Asakura M;Sasaki K;Uematsu Y;Mieki A;Kataoka H;Kawai T
• 通讯作者: Kawai T

Bone-like tissue induced by rhBMP-2, rhBMP-4 and rhBMP-7 in vitro has ossification potential after implantation.

rhBMP-2、rhBMP-4和rhBMP-7体外诱导的骨样组织在植入后具有骨化潜力。

• 发表时间: 2012
• 作者: T. Hayashi;S. kobayashi;A. Mieki;H. Kataoka;T. Kawai.
• 通讯作者: T. Kawai.

rhBMPs induce immature muscular tissue to differentiate into bone-like tissue.

rhBMP 诱导未成熟的肌肉组织分化为骨样组织。

• 发表时间: 2010
• 作者: Hayashi;T.
• 通讯作者: T.

Promotion of rat calvarial defect healing by induced bone-like tissue

诱导骨样组织促进大鼠颅骨缺损愈合

• 发表时间: 2012
• 作者: 小野瞳; 的場あつ子 ;渡辺泰輔;正木英二;Tatsuhide Hayashi
• 通讯作者: Tatsuhide Hayashi