Development of next-generation therapy for temporomandibular joint arthrosis by gene transfer technique using nanofullerene and ultrasonic wave

利用纳米富勒烯和超声波的基因转移技术开发下一代颞下颌关节关节疗法

• 批准号: 22792004
• 负责人: BASUGI Akihiko
• 金额: $ 1.33万
• 依托单位: Kyushu Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22792004/
• 关键词: ソノポレーション; フラーレン; ナノバブル; 遺伝子導入; 顎関節炎; マイクロバブル

We develop the next-generation therapy for temporomandibular joint arthrosis by gene transfer techmique using the nanofullerene and ultrasonic wave. HIG-82 cells were seeded into silicon chambers. The chambers were attached to a stretching apparatus which applied a uniaxial sinusoidal stretching force. The amounts of IL-1β, IL-1ra, PGE2 released into the culture medium were determined using ELISA. Nanofullerene pretreatment suppressed PGE2 synthesis induced by mechanical stretch. We used sonoporation to transfect DNA-nanofullerene into HIG-82 cells and examined the transfection efficiency. Transfection efficiency was significantly increased when the cells were ultrasonicated with nanofullerene.

我们利用纳米富勒烯和超声波的基因转移技术开发了下一代颞下颌关节关节病的治疗方法。将HIG-82细胞接种到硅室中。将腔室连接到施加单轴正弦拉伸力的拉伸装置。ELISA法测定IL-1β、IL-1 ra、PGE 2的释放量。纳米富勒烯预处理抑制机械拉伸诱导的PGE 2合成。我们用声孔法将DNA-纳米富勒烯转染入HIG-82细胞，并检测转染效率。当用纳米富勒烯超声处理细胞时，转染效率显著增加。

项目成果

下顎骨中心性巨細胞肉芽腫の1例

下颌骨中央巨细胞肉芽肿一例

• 发表时间: 2011
• 期刊: 日本口腔外科学会雑誌
• 作者: 馬杉亮彦;吉岡泉;鶴島弘基;石川文隆;松尾拡;冨永和宏
• 通讯作者: 冨永和宏

本邦における下顎非対称治療の実態調査

日本下颌不对称治疗现状调查

• 发表时间: 2010
• 作者: 馬杉亮彦;土生学;他
• 通讯作者: 他

A novel modification in combined Le Fort I and horseshoe osteotomy for posterior repositioning of the maxilla

Le Fort I 和马蹄形截骨术相结合的新颖改良，用于上颌骨后部重新定位

• 发表时间: 2011
• 期刊: Asian Journal of Oral and Maxillofacial Surgery
• 作者: Yoshioka,I.,Amit Khanal.,Kodama;M.,Habu,M.;Nishikawa,T.,Iwanaga;K.;Kokuryo;S.;Basugi;A.,Sakoda;S.,Fukuda;J.and Tominaga,K.
• 通讯作者: J.and Tominaga,K.