Metabolisms of extracellular matrix in temporomandibular joint

颞下颌关节细胞外基质的代谢

• 批准号: 22792002
• 负责人: ARIYOSHI Wataru
• 金额: $ 2.58万
• 依托单位: Kyushu Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22792002/
• 关键词: 顎関節; 細胞外マトリックス; アグリカナーゼ; ヒアルロン酸; 変形性関節症; アグリカン; ADAMTS4; ADAMTS5; 関節軟骨

Hyaluronan(HA) oligosaccharides serve as competitive receptor antagonists to displace HA from the cell surface and induce cell signaling events. We determined changes in the expression and function of aggrecanases after disruption of chondrocyte CD44.HA interactions. Disruption of chondrocyte CD44.HA interactions with HA oligosaccharides induced the transcription of ADAMTS-4 and ADAMTS-5.The association of GPI. anchored MT4-MMP with ADAMTS-4 was also induced in articular chondrocytes by HA oligosaccharides. Inhibition of the NF-kappa B pathway blocked HA oligosaccharides-mediated stimulation of aggrecanases. These results suggest that disruptive changes in chondrocyte. matrix interactions by HA oligosaccharides induce matrix degradation and elevate aggrecanases via the activation of the NF-kappa B signaling pathway.

透明质酸（HA）寡糖作为竞争性受体拮抗剂，从细胞表面置换HA并诱导细胞信号传导事件。我们确定了软骨细胞CD44.HA相互作用破坏后聚集蛋白聚糖酶的表达和功能的变化。破坏软骨细胞CD44.HA与HA寡糖的相互作用可诱导ADAMTS-4和ADAMTS-5的转录。HA寡糖还诱导关节软骨细胞中具有ADAMTS-4的锚定MT4-MMP。NF-κ B途径的抑制阻断了HA寡糖介导的聚集蛋白聚糖酶刺激。这些结果表明软骨细胞的破坏性变化。HA寡糖与基质的相互作用诱导基质降解，并通过NF-κ B信号通路的激活提高聚集蛋白聚糖酶。

项目成果

Mechanisms involved in enhancement of the expression and function of aggrecanases by hyaluronan oligosaccharides.

• DOI: 10.1002/art.33329
• 发表时间: 2012-01
• 期刊: ARTHRITIS AND RHEUMATISM
• 作者: Ariyoshi, Wataru;Takahashi, Nobunori;Hida, Daisuke;Knudson, Cheryl B.;Knudson, Warren
• 通讯作者: Knudson, Warren

Hyaluronic acid induces osteogenesis in periosteal cell

透明质酸诱导骨膜细胞成骨

• 发表时间: 2010
• 作者: SHO MITSUGI;WATARU ARIYOSHI;TOSHINORI OKINAGA;TETSU TAKAHASHI;TATSUJI NISHIHARA
• 通讯作者: TATSUJI NISHIHARA

Hyaluronic acid enhances BMP-2 induced osteogenesis in MG63 cells

透明质酸增强 BMP-2 诱导的 MG63 细胞成骨

• 发表时间: 2010
• 作者: MICHINAO KAWANO;WATARU ARIYOSHI;KENJIRO IWANAGA;TOSHINORI OKINAGA;MANABU HABU;KAZUHIRO TOMINAGA;TATSUJI NISHIHARA
• 通讯作者: TATSUJI NISHIHARA

Hyaluronan oligosaccharide up-regulate aggrecanase expression and function

透明质酸寡糖上调聚集蛋白聚糖酶的表达和功能

• 发表时间: 2011
• 作者: WATARU ARIOSHI;TOSHINORI OKINAGA;TATSUJI NISHIHARA
• 通讯作者: TATSUJI NISHIHARA

Purified fraction of EMD inhibits the proliferation of epithelial cells

EMD 的纯化部分抑制上皮细胞的增殖

• 发表时间: 2010
• 作者: SACHIKO FUJIMOTO;NORIKO SAITO;WATARU ARIYOSHI;TOSHINORI OKINAGA;KAZUNORI YAMAGUCHI;TATSUJI NISHIHARA
• 通讯作者: TATSUJI NISHIHARA