Examination of intracellular localization and transport pathway of bone sialoprotein.

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Bone sialoprotein(BSP) is expected one of the important protein for calcification of bone. In order to clear the mechanisms of it through BSP, we investigated regulation of BSP transcription and transport pathway of BSP proteins in cell. Here, after stimulation with bone morphogenetic protein 2(BMP-2) of C2C12 cells which were myoblast, we showed that Smad1 bound to HOX domain in BSP promoter sequence and that Smad1 and Runx2 bound to FRE domain. And also we detected that C2C12 cells expressed BSP protein to induce calcification. Now, we are observing intracellular localization in the cells by electron microscopy.