Regeneration of a large-scale and higher-order structural tissue using mesenchymal stem cells under culturing technique of heterogeneous mechanical circumstance

异质机械环境培养技术下间充质干细胞再生大规模高阶结构组织

• 批准号: 22760073
• 负责人: MORITA Yasuyuki
• 金额: $ 2.66万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22760073/
• 关键词: 間葉系幹細胞; 腱細胞・腱組織; 細胞外マトリックス; コラーゲンネットワーク; 機械的伸縮刺激; 細胞配列・配向; ひずみ勾配; 許容限界軸ひずみ; I型コラーゲン; テネイシン; 力学的刺激; ひずみ; 腱組織; 腱細胞

The following conclusions were obtained in this study. When mesenchymal stem cells(hBMSCs) were subjected to uniform stretching as mechanical stimulation, there was different strain relativity between amounts of protein synthesis, based on the extracellular matrix, and the tissue structuralization. In addition, an allowable limit axial strain of hBMSCs which directly related to the cell alignment behavior, was elucidated simply and precisely using heterogeneous strain stimulation technique. The axial strain value was 5. 1% in the tensile stimulation. Furthermore, synthesis curve of respective proteins was strongly dependent upon the stretch ratio. Type I collagen synthesis indicated broadband responsiveness in terms of the stretch ratio.

本研究得出了以下结论。当间充质干细胞作为机械刺激受到均匀拉伸时，基于细胞外基质的蛋白质合成量与组织组织化之间存在不同的应变相关性。此外，利用非均相应变刺激技术，简单而准确地阐明了与细胞排列行为直接相关的hBMSCs的允许极限轴向应变。拉伸刺激时的轴向应变值为5.1%。此外，各蛋白质的合成曲线强烈依赖于拉伸比。I型胶原合成在拉伸比方面显示出宽带反应。

项目成果

Deformation measurement of bonemodel with a dental implant by DIC

DIC 测量种植牙骨模型的变形

• 发表时间: 2010
• 作者: Y. Morita;Y. Matsushita;M. Todo;and K. Koyano
• 通讯作者: and K. Koyano

間葉系幹細胞に対する伸縮刺激が細胞外マトリックスの発現量と構造におよぼす影響

拉伸刺激对间充质干细胞表达水平及细胞外基质结构的影响

• 发表时间: 2012
• 期刊: 日本機械学会講演論文集
• 作者: 鈴木敏;et al
• 通讯作者: et al

Shrinkage analysis of a light-cured composite resin in cavity by X-ray CT images

X 射线 CT 图像分析光固化复合树脂型腔内的收缩情况

• 发表时间: 2010
• 期刊: Journal of JSEM
• 作者: K. Arakawa;T. Furukawa;Y. Morita;M. Uchino;and H. Kaida
• 通讯作者: and H. Kaida

Interface Oral Health 2011

界面口腔健康 2011

• 发表时间: 2011
• 作者: Yasuyuki Morita;et al
• 通讯作者: et al

Interface oral health science 2011

• DOI: 10.1007/978-4-431-54070-0
• 发表时间: 2012
• 作者: K. Sasaki;O. Suzuki;N. Takahashi;P. Stashenko;M. Cambridge