Regeneration of a large-scale and higher-order structural tissue using mesenchymal stem cells under culturing technique of heterogeneous mechanical circumstance

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The following conclusions were obtained in this study. When mesenchymal stem cells(hBMSCs) were subjected to uniform stretching as mechanical stimulation, there was different strain relativity between amounts of protein synthesis, based on the extracellular matrix, and the tissue structuralization. In addition, an allowable limit axial strain of hBMSCs which directly related to the cell alignment behavior, was elucidated simply and precisely using heterogeneous strain stimulation technique. The axial strain value was 5. 1% in the tensile stimulation. Furthermore, synthesis curve of respective proteins was strongly dependent upon the stretch ratio. Type I collagen synthesis indicated broadband responsiveness in terms of the stretch ratio.