Protein confomational dynamics probed by plasmon-coupling of nanoparticle

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A measurement technique for reaction dynamics of proteins by an electronic coupling of localized surface plasmon resonance (LSPR) of individual gold nanoparticle was exploited. An apparatus which consists of dark-field microscope equipped with high-sensitive CMOS camera was constructed and employed for the measurements. A plasmon-coupling between single pairs of gold nanoparticles, which were modified with some proteins, was examined. The Light-scattering signal attribute to plasmon-coupling was digitally modulated with the observation time, which indicates the observed phenomena correspond to single-molecule events.