Development of a highly sensitive visual immunoassay method based on DNA amplification and gold nanoparticles.

免疫分析法因其特异性而成为最重要的分析方法之一。为了简化分析，迫切需要一种基于高灵敏度视觉测试的新方法。我们建立了利用酶和无机材料（金纳米颗粒）和生物分子（DNA）的偶联物进行免疫测定的基本技术。我们研究开发固体底物作为高效反应场，制备DNA修饰抗体，优化金纳米颗粒与DNA样品扩增产物的反应。

Immunoassay is one of the most important analytical methods because of its specificity. To simplify the analysis, a new method based on highly sensitive visual test is strongly required. We established fundamental technologies for immunoassays using enzymes and conjugates of inorganic materials (gold nanoparticle) and biomolecules (DNA). We investigated to develop solid substrates used as efficient reaction fields, make DNA-modified antibodies, and optimize the reactions of gold nanoparticles and amplified products of DNA samples.