Construction of self-assembled protein-based microsphere having molecular recognition ability.

我们利用两亲性蛋白、疏水蛋白构建了自组装膜和自组装颗粒。然后，我们建立了基于酶的锚定方法，将生物分子如DNA适体、肽和蛋白质与基于两亲性蛋白质的自组装膜和粒子连接起来。该体系可用于构建具有分子识别能力的蛋白质微球。此外，该蛋白质基微球可应用于由生物标志物分子在人体内诱导的主动药物释放系统。

We constructed self-assembled membranes and particles using amphipathic protein, hydrophobins. We, then, established the enzyme based anchoring methods to connect biological molecules, such as DNA aptamer, peptide and protein, with amphipathic protein-based self-assembly membrane and particle. The system can be utilized for construction protein microsphere having molecular recognition ability. Furthermore, this protein based microsphere can be applied for an active drug release system induced by biomarker molecule in human body.