Concerted regulation of chromosome-templated processes by histones and multi-functional transcription factors

染色体DNA是一个动态的分子，它被复制、转录和重组，但它被包装成一个高度浓缩的结构，称为染色质。我们感兴趣的是，在染色质环境中，这些过程是如何协调的，以便在适当的时间发生适当的反应。我们以分裂酵母为模式生物，分析了atf1-PCR1和组蛋白在转录和同源重组中的功能。前者是DNA结合转录因子的复合体，后者是构成染色质的最丰富的蛋白质。我们的研究结果概括如下：(1)我们已经确定了atf1的转录激活结构域。我们还发现该结构域不仅参与转录，还参与重组。(2)我们比较了依赖于atf1-PCR1的重组位点和依赖于atf1-PCR1的转录起始点的组蛋白修饰模式。重组与组蛋白H3lyse9(H3K9ac)的乙酰化有关，转录与H3K9ac、H3lysine14的乙酰化和H3lysine4的三甲基化相关。(3)我们还发现H3K9ac与重组有关，并且对重组是重要的，与atf1-PCR1定位无关。

Chromosomal DNA is a dynamic molecule that is replicated, transcribed, andrecombined, yet it is packaged into a highly condensed structure termed chromatin. We are interested in how, in chromatin environment, these processes are coordinated so that an appropriate reaction occur at an appropriate timing.We used fission yeast as a model organism, and analyzed functions of Atf1-Pcr1 and histones in transcription and homologous recombination. The former is a complex of DNA-binding transcription factors, and the latter is the most abundant protein that constitutes chromatin. Our findings are summarized as follows.(1) We have identified the transcriptional activation domain of Atf1. We also showed that this domain is involved not only in transcription, but also in recombination.(2) We compared histone modification patterns at Atf1-Pcr1 dependent recombination steis and Atf1-Pcr1 dependent transcriptional start site. While recombination was associated with acetylation of histone H3 lyse9 (H3K9ac), transcription was associated with H3K9ac, acetylation of H3lysine14, and trimethylation of H3 lysine4. (3) We also showed that H3K9ac is associated with and is important for recombination, regardless of Atf1-Pcr1 localization.