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Concerted regulation of chromosome-templated processes by histones and multi-functional transcription factors

组蛋白和多功能转录因子对染色体模板过程的协同调节

基本信息

批准号：
22770003
负责人：
YAMADA Takatomi
金额：
$ 2.66万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Young Scientists (B)
财政年份：
2010
资助国家：
日本
起止时间：
2010 至 2012
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22770003/
关键词：
ヒストン多機能性転写因子染色体

项目摘要

Chromosomal DNA is a dynamic molecule that is replicated, transcribed, andrecombined, yet it is packaged into a highly condensed structure termed chromatin. We are interested in how, in chromatin environment, these processes are coordinated so that an appropriate reaction occur at an appropriate timing.We used fission yeast as a model organism, and analyzed functions of Atf1-Pcr1 and histones in transcription and homologous recombination. The former is a complex of DNA-binding transcription factors, and the latter is the most abundant protein that constitutes chromatin. Our findings are summarized as follows.(1) We have identified the transcriptional activation domain of Atf1. We also showed that this domain is involved not only in transcription, but also in recombination.(2) We compared histone modification patterns at Atf1-Pcr1 dependent recombination steis and Atf1-Pcr1 dependent transcriptional start site. While recombination was associated with acetylation of histone H3 lyse9 (H3K9ac), transcription was associated with H3K9ac, acetylation of H3lysine14, and trimethylation of H3 lysine4. (3) We also showed that H3K9ac is associated with and is important for recombination, regardless of Atf1-Pcr1 localization.

染色体DNA是一种动态的分子，可以复制、转录和重组，但它被包装成一种高度浓缩的结构，称为染色质。本研究以裂殖酵母为模式生物，分析了Atf 1-Pcr 1和组蛋白在转录和同源重组中的功能。前者是DNA结合转录因子的复合物，后者是构成染色质的最丰富的蛋白质。我们的研究结果总结如下。(1)我们已经确定了Atf 1的转录激活域。我们还发现，该结构域不仅参与转录，而且还参与重组。(2)我们比较了Atf 1-Pcr 1依赖性重组位点和Atf 1-Pcr 1依赖性转录起始位点的组蛋白修饰模式。虽然重组与组蛋白H3裂解酶9（H3 K9 ac）的乙酰化有关，但转录与H3 K9 ac、H3赖氨酸14的乙酰化和H3赖氨酸4的三甲基化有关。(3)我们还表明，H3 K9 ac是相关的，是重要的重组，无论Atf 1-Pcr 1定位。